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Literature summary extracted from

  • Jiang, Y.; Yang, X.; Liu, B.; Zhao, H.; Cheng, Q.; Cai, B.
    Catechol 2,3-dioxygenase from Pseudomonas sp. strain ND6: Gene sequence and enzyme characterization (2004), Biosci. Biotechnol. Biochem., 68, 1798-1800.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli DH 5alpha Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.011
-
 catechol 57°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 36000
-
 SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas sp. cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas sp. ND6 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp.
-
-
-
1.13.11.2 Pseudomonas sp. ND6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2 recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 catechol + O2
-
 Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas sp. ND6 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway Pseudomonas sp. ND6 2-hydroxymuconate semialdehyde
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 57
-
 63% loss of activity within 10 min Pseudomonas sp.