Any feedback?
Literature summary extracted from
- Deoxysarpagine hydroxylase--a novel enzyme closing a short side pathway of alkaloid biosynthesis in Rauvolfia (2002), Bioorg. Med. Chem., 10, 2479-2483.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.136 | Ancymidole | 1.0 mM, 49% inhibition | Rauvolfia serpentina |  |
| 1.14.14.136 | BAS 110 W | 1 mM, complete inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | BAS 111 W | 1 mM, 84% inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | CO | ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light | Rauvolfia serpentina | |
| 1.14.14.136 | cytochrome c | 0.01 mM, 46% inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | ketoconazole | 1 mM, 69% inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | LAB 1500978 | 1 mM, 74% inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | Metyrapone | 1 mM, 83% inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | NADP+ | competitive inhibition | Rauvolfia serpentina | |
| 1.14.14.136 | Tetcyclacis | 1 mM, 45% inhibition | Rauvolfia serpentina | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.136 | 0.0074 | - |
10-deoxysarpagine | 35°C, pH 8.0 | Rauvolfia serpentina | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.136 | membrane | associated | Rauvolfia serpentina | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.136 | 10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2 | Rauvolfia serpentina | most probably the natural substrate | sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.136 | Rauvolfia serpentina | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.136 | - |
Rauvolfia serpentina |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.136 | -25°C, 20% sucrose, more than 3 months, no loss of activity | Rauvolfia serpentina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.136 | 10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rauvolfia serpentina | sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.136 | 10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2 | most probably the natural substrate | Rauvolfia serpentina | sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.136 | 35 | - |
- |
Rauvolfia serpentina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.136 | 8 | - |
- |
Rauvolfia serpentina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
