BRENDA - Enzyme Database

Deoxysarpagine hydroxylase--a novel enzyme closing a short side pathway of alkaloid biosynthesis in Rauvolfia

Yu, B.; Ruppert, M.; Stockigt, J.; Bioorg. Med. Chem. 10, 2479-2483 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.136
Ancymidole
1.0 mM, 49% inhibition
Rauvolfia serpentina
1.14.14.136
BAS 110 W
1 mM, complete inhibition
Rauvolfia serpentina
1.14.14.136
BAS 111 W
1 mM, 84% inhibition
Rauvolfia serpentina
1.14.14.136
CO
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
Rauvolfia serpentina
1.14.14.136
cytochrome c
0.01 mM, 46% inhibition
Rauvolfia serpentina
1.14.14.136
ketoconazole
1 mM, 69% inhibition
Rauvolfia serpentina
1.14.14.136
LAB 1500978
1 mM, 74% inhibition
Rauvolfia serpentina
1.14.14.136
Metyrapone
1 mM, 83% inhibition
Rauvolfia serpentina
1.14.14.136
NADP+
competitive inhibition
Rauvolfia serpentina
1.14.14.136
Tetcyclacis
1 mM, 45% inhibition
Rauvolfia serpentina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.136
0.0074
-
10-deoxysarpagine
35°C, pH 8.0
Rauvolfia serpentina
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.136
membrane
associated
Rauvolfia serpentina
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
most probably the natural substrate
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.136
Rauvolfia serpentina
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.14.14.136
-
Rauvolfia serpentina
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Storage Stability
EC Number
Storage Stability
Organism
1.14.14.136
-25°C, 20% sucrose, more than 3 months, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
-
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
most probably the natural substrate
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.136
35
-
-
Rauvolfia serpentina
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.136
8
-
-
Rauvolfia serpentina
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.136
Ancymidole
1.0 mM, 49% inhibition
Rauvolfia serpentina
1.14.14.136
BAS 110 W
1 mM, complete inhibition
Rauvolfia serpentina
1.14.14.136
BAS 111 W
1 mM, 84% inhibition
Rauvolfia serpentina
1.14.14.136
CO
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
Rauvolfia serpentina
1.14.14.136
cytochrome c
0.01 mM, 46% inhibition
Rauvolfia serpentina
1.14.14.136
ketoconazole
1 mM, 69% inhibition
Rauvolfia serpentina
1.14.14.136
LAB 1500978
1 mM, 74% inhibition
Rauvolfia serpentina
1.14.14.136
Metyrapone
1 mM, 83% inhibition
Rauvolfia serpentina
1.14.14.136
NADP+
competitive inhibition
Rauvolfia serpentina
1.14.14.136
Tetcyclacis
1 mM, 45% inhibition
Rauvolfia serpentina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.136
0.0074
-
10-deoxysarpagine
35°C, pH 8.0
Rauvolfia serpentina
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.136
membrane
associated
Rauvolfia serpentina
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
most probably the natural substrate
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.136
-
Rauvolfia serpentina
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.14.136
-25°C, 20% sucrose, more than 3 months, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
-
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.136
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
most probably the natural substrate
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.136
35
-
-
Rauvolfia serpentina
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.136
8
-
-
Rauvolfia serpentina