Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, X.; Qin, W.; Lehotay, M.; Toki, D.; Dennis, P.; Schutzbach, J.S.; Brockhausen, I.
    Soluble human core 2 beta6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity (2003), Biochim. Biophys. Acta, 1648, 62-74.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.102 2-mercaptoethanol stimulates up to 20fold Homo sapiens
2.4.1.102 dithiothreitol stimulates up to 20fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.102 expression of the soluble catalytic domain in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.102 C100S mutant enzyme with 10% of wild-type activity Homo sapiens
2.4.1.102 C151S inactive mutant protein Homo sapiens
2.4.1.102 C172S inactive mutant protein Homo sapiens
2.4.1.102 C199S inactive mutant protein Homo sapiens
2.4.1.102 C217S mutant enzyme with 41% of wild-type activity. Similar Km and Vmax values for Galbeta(1-3)GalNAcalpha-p-nitrophenol, Km-value for UDP-N-acetyl-D-glucosamine is 2.9fold higher than wild-type value. In contrast to wild-type enzyme, the mutant enzyme is not inhibited by sulfhydryl reagents and is not stimulated by reducing agents Homo sapiens
2.4.1.102 C372S inactive mutant protein Homo sapiens
2.4.1.102 C381S inactive mutant protein Homo sapiens
2.4.1.102 C413S inactive mutant protein Homo sapiens
2.4.1.102 C59S inactive mutant protein Homo sapiens
2.4.1.102 DELTA407-428 inactive mutant protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.102 5,5'-dithiobis-2-nitrobenzoic acid inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 1 mM inhibits 94% Homo sapiens
2.4.1.102 diethyldicarbonate 0.2 mM, 74% inhibition Homo sapiens
2.4.1.102 Hydroxyphenylglyoxal 19% inhibition at 0.25 mM, complete inhibition at 5-10 mM Homo sapiens
2.4.1.102 iodoacetate 0.2 mM inhibits the enzyme 70% Homo sapiens
2.4.1.102 additional information enzyme is fully active in presence of 5 mM EDTA Homo sapiens
2.4.1.102 N-bromosuccinimide 5 mM, complete inhibition Homo sapiens
2.4.1.102 NEM inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 0.2 mM inhibits 42% Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.102 1.1
-
Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 7, 37°C, wild-type enzyme Homo sapiens
2.4.1.102 1.6
-
Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 7, 37°C, mutant enzyme C217S Homo sapiens
2.4.1.102 1.9
-
UDP-N-acetyl-D-glucosamine pH 7, 37°C, wild-type enzyme Homo sapiens
2.4.1.102 5.6
-
UDP-N-acetyl-D-glucosamine pH 7, 37°C, mutant enzyme C217S Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.102 additional information no requirement for metal ions Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.102 58000
-
1 * 58000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Homo sapiens
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.102 -80°C, stable for at least 6 months, wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GalNAcalpha-p-nitrophenol
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.102 monomer 1 * 58000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.102 C2GnT1
-
Homo sapiens
2.4.1.102 core 2 beta6-N-acetylglucosaminyltransferase
-
Homo sapiens