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Literature summary extracted from

  • Hiol, A.; Caron, J.M.; Smith, C.D.; Jones, T.L.
    Characterization and partial purification of protein fatty acyltransferase activity from rat liver (2003), Biochim. Biophys. Acta, 1635, 10-19.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.142 enzyme is inactivated by degrading or denaturaring agents, instable during final gel filtration purification step Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.142 hydroxylamine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.142 plasma membrane
-
 Rattus norvegicus 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.142 additional information Rattus norvegicus acylation of proteins through the addition of palmitate to a cysteine residue is a common posttranslational modification for a variety of proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.142 Rattus norvegicus
-
 male CD strain rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.142 partial, 95fold from liver by preparation of plasma membrane fraction, solubilization with 0.15% Triton X-100, and sequential chromatography including affinity chromatography on a cerulin-based palmitoylation inhibitor ligand Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.142 liver
-
 Rattus norvegicus
-
2.3.1.142 additional information the addition of different long-chain fatty acids to proteins is cell-type specific Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.142 2.4
-
 liver plasma membrane preparation Rattus norvegicus
2.3.1.142 228
-
 partially purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.142 -80°C, partially purified enzyme, 0.15% v/v Triton X-100, 150 mM KCl, 20 mM Tris-HCl, pH 7.4, 1 mM EGTA, 0.01 mg/ml leupeptin, at least 3 months without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.142 additional information acylation of proteins through the addition of palmitate to a cysteine residue is a common posttranslational modification for a variety of proteins Rattus norvegicus ?
-
 ?
2.3.1.142 additional information the enzyme shows a preference for palmitate and to a lesser degree for other long-chain fatty acids, overview Rattus norvegicus ?
-
 ?
2.3.1.142 RGS16 + palmitoyl-CoA RGS16 is the regulator of G protein signaling 16 Rattus norvegicus O-palmitoyl-RGS16 + CoA
-
 r
2.3.1.142 tubulin Galphai + palmitoyl-CoA
-
 Rattus norvegicus O-palmitoyl-tubulin Galphai + CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.142 Pat
-
 Rattus norvegicus
2.3.1.142 PATI
-
 Rattus norvegicus
2.3.1.142 protein fatty acyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.142 30
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.142 95
-
 5 min, partial inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.142 7.4
-
 assay at Rattus norvegicus