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Literature summary extracted from

  • Zhang, L.; Li, Z.; Quinn, B.; Yu, L.; Yu, C.A.
    Nonoxidizable ubiquinol derivatives that are suitable for the study of the ubiquinol oxidation site in the cytochrome bc1 complex (2002), Biochim. Biophys. Acta, 1556, 226-232.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.1.1.8 purified cytochrome bc1 complex with bound inhibitors 2,3,4-trimethoxy-5-decyl-6-methyl-phenol or 2,3,4-trimethoxy-5-methyl-6-decyl-phenol, the latter in a 4fold molar excess, 2-4 weeks, X-ray diffraction structure determination and analysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.8 2,3,4-trimethoxy-5-decyl-6-methyl-phenol competitive inhibitor, binds stably to the Qo site of the cytochrome bc1 complex, binding structure, inhibitory potency depends on the chain length of the ubiquinol substrate and is higher with Q0C5 than with Q0C10, inhibition is pH-dependent Bos taurus
7.1.1.8 2,3,4-trimethoxy-5-methyl-6-decyl-phenol competitive inhibitor, binds stably to the Qo site of the cytochrome bc1 complex, binding structure, inhibition is pH-dependent Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.8 0.02
-
 ubiquinol pH 7.4, 25°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.8 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.8 ubiquinol-2 + ferricytochrome c Bos taurus the cytochrome bc1 complex is the central segment of the respiratory chain in mitochondria ubiquinone-2 + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.8 Bos taurus
-
 enzyme is part of the cytochrome bc1 complex
-

Reaction

EC Number Reaction Comment Organism Reaction ID
7.1.1.8 quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+[side 2] substrate binding mechanism at the Qo site of the cytochrome bc1 complex Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.8 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.8 ubiquinol-2 + ferricytochrome c the cytochrome bc1 complex is the central segment of the respiratory chain in mitochondria Bos taurus ubiquinone-2 + ferrocytochrome c
-
 ?
7.1.1.8 ubiquinol-2 + ferricytochrome c substrate of chain length C5 to C10, ubiquinol binds transiently at the Qo site, only when both heme bL and the iron sulfur cluster are in the oxidized form, where it is oxidized Bos taurus ubiquinone-2 + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.8 More enzyme is part of the mitochondrial cytochrome bc1 complex Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.1.1.8 25
-
 assay at, cytochrome bc1 complex Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.8 8
-
 cytochrome bc1 complex Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.1.1.8 6.5 9 cytochrome bc1 complex Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.1.1.8 0.0068
-
 2,3,4-trimethoxy-5-decyl-6-methyl-phenol pH 7.4, 25°C Bos taurus
7.1.1.8 0.0716
-
 2,3,4-trimethoxy-5-methyl-6-decyl-phenol pH 7.4, 25°C Bos taurus