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Literature summary extracted from

  • Zavilgelsky, G.B.; Kotova, V.Y.; Mazhul, M.M.; Manukhov, I.V.
    Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells (2002), Biochemistry (Moscow), 67, 986-992.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.3 additional information the enzyme shows enhanced affinity for chaperone ClpA Photorhabdus luminescens
1.14.14.3 additional information the enzyme shows enhanced affinity for chaperone DnaK Aliivibrio fischeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively Aliivibrio fischeri
1.14.14.3 expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively Photorhabdus luminescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 RCHO + FMNH2 + O2 Aliivibrio fischeri 3-step process via H2O2 as intermediate RCOOH + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir
1.14.14.3 RCHO + FMNH2 + O2 Photorhabdus luminescens 3-step process via H2O2 as intermediate RCOOH + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Aliivibrio fischeri
-
marine bacterium
-
1.14.14.3 Photorhabdus luminescens
-
terrestrial bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.3 recombinant enzyme from Escherichia coli Aliivibrio fischeri
1.14.14.3 recombinant enzyme from Escherichia coli Photorhabdus luminescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.14.3 quick refolding within several min at room temperature or 25°C of thermoinactivated enzyme requires ATP and the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone but is independent of chaperone ClpA, 80% activity after reactivation, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview Aliivibrio fischeri
1.14.14.3 slow refolding at room temperature or 35°C of thermoinactivated recominant enzyme requires the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone, 7-8% activity after refolding over 10 min, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview Photorhabdus luminescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 n-decanal + FMNH2 + O2 3-step process via H2O2 as intermediate Aliivibrio fischeri n-decanoate + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir
1.14.14.3 n-decanal + FMNH2 + O2 3-step process via H2O2 as intermediate Photorhabdus luminescens n-decanoate + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir
1.14.14.3 RCHO + FMNH2 + O2 3-step process via H2O2 as intermediate Aliivibrio fischeri RCOOH + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir
1.14.14.3 RCHO + FMNH2 + O2 3-step process via H2O2 as intermediate Photorhabdus luminescens RCOOH + FMN + H2O + hn generation of blue-green light of wavelength 490 nm ir

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 bacterial luciferase
-
Aliivibrio fischeri
1.14.14.3 bacterial luciferase
-
Photorhabdus luminescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.3 25
-
-
Aliivibrio fischeri
1.14.14.3 35
-
-
Photorhabdus luminescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 additional information
-
the recombinant enzyme shows reduced thermal stability in absence of chaperone Hsp100 after expression in an Escherichia coli clpA-mutant strain, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo Photorhabdus luminescens
1.14.14.3 additional information
-
the recombinant enzyme shows unaffected thermal stability after expression in an Escherichia coli clpA-mutant strain lacking chaperone Hsp100, carbonyl cyanide 3-chlorophenylhydrazone highly decreases the thermal stability of the enzyme in vivo to in vitro level, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo Aliivibrio fischeri
1.14.14.3 43.5
-
inactivation in vitro, temperature-stable enzyme Photorhabdus luminescens
1.14.14.3 43.5
-
inactivation, temperature-labile enzyme Aliivibrio fischeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.3 7
-
assay at Aliivibrio fischeri
1.14.14.3 7
-
assay at Photorhabdus luminescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 FMNH2
-
Aliivibrio fischeri
1.14.14.3 FMNH2
-
Photorhabdus luminescens