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Literature summary extracted from

  • Efimov, I.; Cronin, C.N.; Bergmann, D.J.; Kuusk, V.; McIntire, W.S.
    Insight into covalent flavinylation and catalysis from redox, spectral, and kinetic analyses of the R474K mutant of the flavoprotein subunit of p-cresol methylhydroxylase (2004), Biochemistry, 43, 6138-6148.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.9.1 expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.17.9.1 R474K reduced rate of FAD binding, strongly reduced activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.9.1 9200
-
 alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida
1.17.9.1 59500
-
 alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.9.1 4-cresol + acceptor + H2O Pseudomonas putida via intermediate 4-hydroxybenzylalcohol 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
1.17.9.1 4-cresol + acceptor + H2O Pseudomonas putida NCIMB 9869 via intermediate 4-hydroxybenzylalcohol 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Pseudomonas putida
-
 NCIMB 9869
-
1.17.9.1 Pseudomonas putida NCIMB 9869
-
 NCIMB 9869
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.9.1 native and mutant enzymes from Escherichia coli Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
1.17.9.1 4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida NCIMB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.9.1 tetramer alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.9.1 2.4
-
 4-Cresol R474K mutant enzyme, pH 7, 25°C Pseudomonas putida
1.17.9.1 121
-
 4-Cresol native enzyme, pH 7, 25°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.9.1 FAD
-
 Pseudomonas putida