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Literature summary extracted from

  • Momany, C.; Levdikov, V.; Blagova, L.; Lima, S.; Phillips, R.S.
    Three-dimensional structure of kynureninase from Pseudomonas fluorescens (2004), Biochemistry, 43, 1193-1203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.3 expression in Escherichia coli Pseudomonas fluorescens
3.7.1.3 subcloned into pSapKO-WT and transformed into Escherichia coli B834(DE3) Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.7.1.3 by hanging-drop vapor diffusion method at room temperature, to 1.85 A resolution, Lys-227 is the pyridoxal-5'-phosphate binding residue near the pyridoxal-5'-phosphate nitrogen, but Asp-132 is also strictly conserved and at a similar distance from the pyridinium nitrogen, Tyr-226 donates a hydrogen bond to the phosphate of pyridoxal-5'-phosphate, Trp-256 donates a hydrogen bond to the phosphate through the indole N1-hydrogen Pseudomonas fluorescens
3.7.1.3 performed using the hanging-drop vapor diffusion technique Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.3 D132A has lower activity and binds pyridoxal 5'-phosphate weakly Pseudomonas fluorescens
3.7.1.3 D132A has lower activity and weaklier pyridoxal-5'-phosphate binding than that of wild-type enzyme Pseudomonas fluorescens
3.7.1.3 D132E has good activity and pyridoxal 5'-phosphate binding stronger than that of the wild-type enzyme Pseudomonas fluorescens
3.7.1.3 D132E has good activity and stronger pyridoxal-5'-phosphate binding than that of wild-type enzyme Pseudomonas fluorescens
3.7.1.3 D201A has very low activity, less than 0.01% of that of the wild-type enzyme, binds pyridoxal 5'-phosphate weakly Pseudomonas fluorescens
3.7.1.3 D201A has very low activity and binds pyridoxal-5'-phosphate weakly, and expresses poorly, giving large amounts of insoluble inclusion bodies and very low levels of soluble protein Pseudomonas fluorescens
3.7.1.3 D201E has good activity and pyridoxal 5'-phosphate binding stronger than that of the wild-type enzyme Pseudomonas fluorescens
3.7.1.3 D201E has good activity and stronger pyridoxal-5'-phosphate binding than that of wild-type enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.3 L-kynurenine + H2O Pseudomonas fluorescens L-tryptophan metabolism anthranilate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.3 Pseudomonas fluorescens P83788
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.7.1.3 no modification
-
 Pseudomonas fluorescens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.3
-
 Pseudomonas fluorescens
3.7.1.3 recombinant enzyme, by centrifugation, protamine sulfate precipitation, HiTrap Q column, omega-aminohexylagarose column, second HiTrap Q column and phenyl-Sepharose column Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.7.1.3 -78ºC, 0.1 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 year, stable Pseudomonas fluorescens
3.7.1.3 -78ºC, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 month, loses 4% of activity Pseudomonas fluorescens
3.7.1.3 4ºC, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 week, loses 40% of activity Pseudomonas fluorescens
3.7.1.3 78°C, phosphate buffer, 1 year Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.3 L-kynurenine + H2O L-tryptophan metabolism Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.3 dimer
-
 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.7.1.3 L-kynurenine hydrolase
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.3 pyridoxal 5'-phosphate
-
 Pseudomonas fluorescens