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Literature summary extracted from

  • Brazeau, B.J.; Lipscomb, J.D.
    Key amino acid residues in the regulation of soluble methane monooxygenase catalysis by component B (2003), Biochemistry, 42, 5618-5631.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.25 T111Y mutant enzyme with increased rate constant for the reaction of large substrates such as ethane, furan, and nitrobenzene with the reactive MMOH (regulatory componant of the enzyme) intermediate Q while decreasing the rate constant for the reaction with methane. The regiospecificity for nitrobenzene oxidation is altered and 10fold more T111Y than wild-type MMOB is required to maximize the rate of turnover Methylosinus trichosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 soluble
-
Methylosinus trichosporium
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium
-
OB3b
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 ethane + NADH + O2
-
Methylosinus trichosporium ethanol + NAD+ + H2O
-
?
1.14.13.25 furan + NADH + O2
-
Methylosinus trichosporium ? + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + O2
-
Methylosinus trichosporium methanol + NAD+ + H2O
-
?
1.14.13.25 nitrobenzene + NADH + O2
-
Methylosinus trichosporium nitrophenol + NAD+ + H2O
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylosinus trichosporium