EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.1 | expression of mutant enzymes in Escherichia coli BL21-Codon Plus | Acinetobacter sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.1 | D218A | no activity | Acinetobacter sp. |
1.14.12.1 | D218E | no activity | Acinetobacter sp. |
1.14.12.1 | D218N | no activity | Acinetobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.1 | 24000 | - |
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
1.14.12.1 | 52000 | - |
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
1.14.12.1 | 220000 | - |
gel filtration, wild type and mutant enzymes | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.1 | anthranilic acid + NAD(P)H + O2 + H2O | Acinetobacter sp. | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
1.14.12.1 | anthranilic acid + NAD(P)H + O2 + H2O | Acinetobacter sp. ADP1 | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | catechol + CO2 + NAD(P)+ + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.1 | Acinetobacter sp. | - |
- |
- |
1.14.12.1 | Acinetobacter sp. ADP1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.12.1 | wild type and mutant enzymes | Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.1 | anthranilic acid + NAD(P)H + O2 + H2O | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | Acinetobacter sp. | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
1.14.12.1 | anthranilic acid + NAD(P)H + O2 + H2O | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | Acinetobacter sp. ADP1 | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
1.14.12.1 | anthranilic acid + NADH + O2 + H2O | - |
Acinetobacter sp. | catechol + CO2 + NAD+ + NH3 | - |
? | |
1.14.12.1 | anthranilic acid + NADH + O2 + H2O | - |
Acinetobacter sp. ADP1 | catechol + CO2 + NAD+ + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.12.1 | hexamer | apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.1 | additional information | - |
oxygenase component completely inactive at pH 9 | Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.1 | iron-sulfur centre | proximal Rieske [2Fe-2S] center | Acinetobacter sp. | |
1.14.12.1 | NADH | - |
Acinetobacter sp. | |
1.14.12.1 | NADPH | - |
Acinetobacter sp. |