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Literature summary extracted from

  • Lin, L.Y.C.; Sulea, T.; Szittner, R.; Kor, C.; Purisima, E.O.; Meighen, E.A.
    Implications of the reactive thiol and the proximal non-proline cis-peptide bond in the structure and function of Vibrio harveyi luciferase (2002), Biochemistry, 41, 9938-9945.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.3 A75G site-directed mutagenesis, activity similar to the wild-type enzyme Vibrio harveyi
1.14.14.3 C106V site-directed mutagenesis, highly reduced ability to stabilize the reaction intermediate due to interaction between Val106 and Ala75 side chains, and therefore highly reduced activity and increased thermal lability compared to the wild-type enzyme Vibrio harveyi
1.14.14.3 C106V/A75G site-directed mutagenesis, mutation of Ala75 restores about 90% of the activity abolished by mutation of Cys106, shift in the light emission spectrum to that of Photobacterium phosphoreum possessing Val and Gly at positions 106 and 75, respectively Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.3 additional information
-
 additional information kinetics, substrate and cofactor binding Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 RCHO + FMNH2 + O2 Vibrio harveyi
-
 RCOOH + FMN + H2O + hn
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.3 a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu reaction mechanism, Cys106 is essential for stabilization of the reaction intermediate, for consumption of aldehyde substrate, and for activity Vibrio harveyi
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.3 additional information
-
 substrate specificity Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH2 + O2
-
 Vibrio harveyi decanoate + FMN + H2O + hn
-
 ir
1.14.14.3 dodecanal + FMNH2 + O2
-
 Vibrio harveyi dodecanoate + FMN + H2O + hn
-
 ir
1.14.14.3 nonanal + FMNH2 + O2
-
 Vibrio harveyi nonanoate + FMN + H2O + hn
-
 ir
1.14.14.3 octanal + FMNH2 + O2
-
 Vibrio harveyi octanoate + FMN + H2O + hn
-
 ir
1.14.14.3 RCHO + FMNH2 + O2
-
 Vibrio harveyi RCOOH + FMN + H2O + hn
-
 ir
1.14.14.3 RCHO + FMNH2 + O2 formation of a C4a-hydroperoxyflavin intermediate Vibrio harveyi RCOOH + FMN + H2O + hn
-
 ir
1.14.14.3 undecanal + FMNH2 + O2
-
 Vibrio harveyi undecanoate + FMN + H2O + hn
-
 ir

Subunits

EC Number Subunits Comment Organism
1.14.14.3 More structure-function relationship, roles of Cys106, Ala75, Ala74, and the isoalloxazine ring of FMN Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 luciferase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.3 23
-
 assay at Vibrio harveyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 40
-
 wild-type enzyme and mutanbt A75G: loss of 60% activity within 50 min, mutants C106V and C106V/A75G show increased thermolability loosing 99% and 90% activity, respectively Vibrio harveyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.3 additional information
-
 additional information single turnover, luminescence Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.3 7
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 FMNH2
-
 Vibrio harveyi