EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | gene luxA, expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Vibrio harveyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.3 | F261A | site-directed mutagenesis, residue of the alpha-subunit, 0.19% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | F261D | site-directed mutagenesis, residue of the alpha-subunit, 0.004% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | F261S | site-directed mutagenesis, residue of the alpha-subunit, 0.13% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | F261Y | site-directed mutagenesis, residue of the alpha-subunit, 2-3% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | G275A | site-directed mutagenesis, residue of the alpha-subunit, 27% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | G275F | site-directed mutagenesis, residue of the alpha-subunit, 6-7% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | G275I | site-directed mutagenesis, residue of the alpha-subunit, 15% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | G275P | site-directed mutagenesis, residue of the alpha-subunit, 0.04% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
1.14.14.3 | G284P | site-directed mutagenesis, residue of the alpha-subunit, 1-2% of the wild-type activity | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.3 | additional information | - |
additional information | stopped flow spectroscopy | Vibrio harveyi | |
1.14.14.3 | 0.0003 | - |
FMNH2 | recombinant wild-type enzyme, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0004 | - |
decanal | recombinant mutant alphaF261S, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0004 | - |
decanal | recombinant mutant alphaG275I, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0006 | - |
FMNH2 | recombinant mutant alphaG284P, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.001 | - |
decanal | recombinant wild-type enzyme, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0016 | - |
decanal | recombinant mutant alphaF261Y, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0017 | - |
decanal | recombinant mutant alphaF261A, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0018 | - |
decanal | recombinant mutant alphaG275P, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0022 | - |
decanal | recombinant mutant alphaG275A, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0022 | - |
FMNH2 | recombinant mutant alphaG275P, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0027 | - |
decanal | recombinant mutant alphaG275F, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0037 | - |
decanal | recombinant mutant alphaG284P, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0046 | - |
decanal | recombinant mutant alphaF261D, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0075 | - |
FMNH2 | recombinant mutant alphaF261D, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0192 | - |
FMNH2 | recombinant mutant alphaF261Y, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0281 | - |
FMNH2 | recombinant mutant alphaF261A, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0358 | - |
FMNH2 | recombinant mutant alphaG275A, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0369 | - |
FMNH2 | recombinant mutant alphaF261S, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0411 | - |
FMNH2 | recombinant mutant alphaG275F, pH 7.0, 25°C | Vibrio harveyi | |
1.14.14.3 | 0.0584 | - |
FMNH2 | recombinant mutant alphaG275I, pH 7.0, 25°C | Vibrio harveyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.3 | recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 to over 85% homogeneity | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | decanal + FMNH2 + O2 | formation of a 4a-hydroperoxy-FMN intermediate II | Vibrio harveyi | decanoate + FMN + H2O + hn | - |
ir | |
1.14.14.3 | RCHO + FMNH2 + O2 | formation of a 4a-hydroperoxy-FMN intermediate II | Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.3 | More | functional roles of conserved residues in the protease-labile, unstructured loop of the alpha-subunit, formed by residues 257-291, the loop undergoed conformational changes during catalysis, overview | Vibrio harveyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | bacterial luciferase | - |
Vibrio harveyi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 25 | - |
assay at | Vibrio harveyi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 7 | - |
assay at | Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.3 | FMNH2 | - |
Vibrio harveyi |