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Literature summary extracted from

  • Sperling, P.; Blume, A.; Zahringer, U.; Heinz, E.
    Further characterization of DELTA8-sphingolipid desaturases from higher plants (2000), Biochem. Soc. Trans., 28, 638-641.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4 expression in Saccharomyces cerevisiae Helianthus annuus
1.14.19.29 expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase Arabidopsis thaliana
1.14.19.29 expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase Brassica napus
1.14.19.29 expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase Helianthus annuus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+ Arabidopsis thaliana
-
 DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+ Brassica napus
-
 DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+ Helianthus annuus
-
 DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+ Helianthus annuus
-
 DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+ Arabidopsis thaliana
-
 DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+ Brassica napus
-
 DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Helianthus annuus
-
-
-
1.14.19.29 Arabidopsis thaliana
-
-
-
1.14.19.29 Brassica napus
-
-
-
1.14.19.29 Helianthus annuus Q43469
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.4 (2S,3R)-2-aminoicosane-1,3-diol + reduced acceptor + O2
-
 Helianthus annuus (E)-(2S,3R)-2-aminoicos-8-ene-1,3-diol + (Z)-(2S,3R)-2-aminoicos-8-ene-1,3-diol + acceptor + H2O
-
 ?
1.14.19.4 phytosphinganine + reduced acceptor + O2
-
 Helianthus annuus trans-8-sphingenine + cis-8-sphingenine + acceptor + H2O
-
 ?
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Arabidopsis thaliana DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Brassica napus DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Helianthus annuus DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Helianthus annuus DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Arabidopsis thaliana DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Brassica napus DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Helianthus annuus
1.14.19.29 DELTA8-sphingolipid desaturase
-
 Arabidopsis thaliana
1.14.19.29 DELTA8-sphingolipid desaturase
-
 Brassica napus
1.14.19.29 DELTA8-sphingolipid desaturase
-
 Helianthus annuus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.4 cytochrome b5 the enzyme is composed of a C-terminal desaturase domain and an N-terminal cytochrome b5 Helianthus annuus
1.14.19.29 cytochrome b5
-
 Arabidopsis thaliana
1.14.19.29 cytochrome b5
-
 Brassica napus
1.14.19.29 cytochrome b5
-
 Helianthus annuus