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Literature summary extracted from

  • Bjoerkhem-Bergman, L.; Jonsson, K.; Eriksson, L.C.; Olsson, J.M.; Lehmann, S.; Paul, C.; Bjornstedt, M.
    Drug-resistant human lung cancer cells are more sensitive to selenium cytotoxicity. Effects on thioredoxin reductase and glutathione reductase (2002), Biochem. Pharmacol., 63, 1875-1884.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.7 doxorubicin differences between drug-sensitive and drug-resistant cells, overview Homo sapiens
1.8.1.7 Selenite 4fold increase in activity in U-1256 cells, less pronounced in presence of doxorubicin, differences between drug-sensitive and drug-resistant cells, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 additional information cytotoxic effect of selenite on drug-sensitive and drug-resistant cancer cell lines, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH Homo sapiens
-
 glutathione + NADP+
-
 ?
1.8.1.7 additional information Homo sapiens drug resistance to doxorubicin or selenite does not influence the enzyme activity in lung cancer cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 GLC-4 cell doxorubicin-sensitive lung cancer cell line Homo sapiens
-
1.8.1.7 GLC-4/ADR cell doxorubicin-insensitive lung cancer cell line expressing the MRP multi-drug resistant protein Homo sapiens
-
1.8.1.7 HL-60 cell doxorubicin-insensitive leukemic cell line HL-60-R, a subclone of HL-60 Homo sapiens
-
1.8.1.7 U-1285 cell doxorubicin-sensitive lung cancer cell line Homo sapiens
-
1.8.1.7 U-1285dox cell doxorubicin-insensitive lung cancer cell line expressing the MRP multi-drug resistant protein Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 additional information
-
 enzyme activity in the different cell lines Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH
-
 Homo sapiens glutathione + NADP+
-
 ?
1.8.1.7 additional information drug resistance to doxorubicin or selenite does not influence the enzyme activity in lung cancer cells Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Homo sapiens
1.8.1.7 GR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH dependent on Homo sapiens