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Literature summary extracted from

  • Gonzalez, B.; Campillo, N.; Garrido, F.; Gasset, M.; Sanz-Aparicio, J.; Pajares, M.A.
    Active-site-mutagenesis study of rat liver betaine-homocysteine S-methyltransferase (2003), Biochem. J., 370, 945-952.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.5 A119G 1.9fold reduced turnover-number for betaine and L-homocysteine, 1.6fold reduced KM-value for L-homocysteine, 1.1fold decrease in Km-value for betaine Rattus norvegicus
2.1.1.5 C186A 1.8fold reduced turnover-number for betaine and L-homocysteine, 4.3fold reduced KM-value for L-homocysteine, 1.8fold increase in Km-value for betaine Rattus norvegicus
2.1.1.5 C186S 2.3fold reduced turnover-number for betaine and L-homocysteine, 1.4fold reduced KM-value for L-homocysteine, 111.6fold decrease in Km-value for betaine Rattus norvegicus
2.1.1.5 D26A 2.7fold reduced turnover-number for betaine and L-homocysteine, 1.7fold reduced KM-value for L-homocysteine, 3.5fold increase in Km-value for betaine Rattus norvegicus
2.1.1.5 D26I 67fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 6.5fold lower than the wild-type value Rattus norvegicus
2.1.1.5 E159G 60fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 61.2fold lower than the wild-type value Rattus norvegicus
2.1.1.5 E159K 170fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 169.7fold lower than the wild-type value Rattus norvegicus
2.1.1.5 E21A 2.3fold reduced turnover-number for betaine and L-homocysteine, 6fold reduced KM-value for L-homocysteine, 2.4fold reduced Km-value for betaine Rattus norvegicus
2.1.1.5 E21K 1.6fold reduced turnover-number for betaine and L-homocysteine, 1.4fold reduced KM-value for L-homocysteine, 1.7fold reduced Km-value for betaine Rattus norvegicus
2.1.1.5 F74A 1.9fold reduced turnover-number for betaine and L-homocysteine, 1.9fold reduced KM-value for L-homocysteine, 2.9fold increase in Km-value for betaine Rattus norvegicus
2.1.1.5 T184G 2.4fold reduced turnover-number for betaine and L-homocysteine, 3.19fold reduced KM-value for L-homocysteine, 1.6fold increase in Km-value for betaine Rattus norvegicus
2.1.1.5 T73G 3.1fold reduced turnover-number for betaine and L-homocysteine, 2.2fold reduced KM-value for L-homocysteine, 1.3fold reduced Km-value for betaine Rattus norvegicus
2.1.1.5 Y77A 14fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 13.7fold lower than the wild-type value Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.017
-
 L-homocysteine 37°C, mutant enzyme E21A Rattus norvegicus
2.1.1.5 0.025
-
 L-homocysteine 37°C, mutant enzyme C186A Rattus norvegicus
2.1.1.5 0.034
-
 L-homocysteine 37°C, mutant enzyme T184G Rattus norvegicus
2.1.1.5 0.049
-
 L-homocysteine 37°C, mutant enzyme T73G Rattus norvegicus
2.1.1.5 0.057
-
 L-homocysteine 37°C, mutant enzyme F74A Rattus norvegicus
2.1.1.5 0.063
-
 L-homocysteine 37°C, mutant enzyme D26A Rattus norvegicus
2.1.1.5 0.068
-
 L-homocysteine 37°C, mutant enzyme A119G Rattus norvegicus
2.1.1.5 0.073
-
 L-homocysteine 37°C, mutant enzyme E21K Rattus norvegicus
2.1.1.5 0.074
-
 L-homocysteine 37°C, mutant enzyme C186S Rattus norvegicus
2.1.1.5 0.106
-
 L-homocysteine 37°C, wild-type enzyme Rattus norvegicus
2.1.1.5 0.139
-
 betaine 37°C, mutant enzyme E21A Rattus norvegicus
2.1.1.5 0.185
-
 betaine 37°C, mutant enzyme C186A Rattus norvegicus
2.1.1.5 0.195
-
 betaine 37°C, mutant enzyme E21K Rattus norvegicus
2.1.1.5 0.206
-
 betaine 37°C, mutant enzyme T186S Rattus norvegicus
2.1.1.5 0.258
-
 betaine 37°C, mutant enzym T73G Rattus norvegicus
2.1.1.5 0.305
-
 betaine 37°C, mutant enzyme A119G Rattus norvegicus
2.1.1.5 0.333
-
 betaine 37°C, wild-type enzyme Rattus norvegicus
2.1.1.5 0.548
-
 betaine 37°C, mutant enzyme T184G Rattus norvegicus
2.1.1.5 0.969
-
 betaine 37°C, mutant enzyme F74A Rattus norvegicus
2.1.1.5 1.17
-
 betaine 37°C, mutant enzyme D26A Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Rattus norvegicus O09171
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 L-homocysteine + betaine
-
 Rattus norvegicus L-methionine + dimethylglycine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.5 0.000082
-
 L-homocysteine 37°C, mutant enzyme E159K Rattus norvegicus
2.1.1.5 0.000082
-
 betaine 37°C, mutant enzyme E159K Rattus norvegicus
2.1.1.5 0.00023
-
 L-homocysteine 37°C, mutant enzyme E159G Rattus norvegicus
2.1.1.5 0.00023
-
 betaine 37°C, mutant enzyme E159G Rattus norvegicus
2.1.1.5 0.001
-
 L-homocysteine 37°C, mutant enzyme Y77A Rattus norvegicus
2.1.1.5 0.001
-
 betaine 37°C, mutant enzyme Y77A Rattus norvegicus
2.1.1.5 0.0021
-
 L-homocysteine 37°C, mutant enzyme D26I Rattus norvegicus
2.1.1.5 0.0021
-
 betaine 37°C, mutant enzyme D26I Rattus norvegicus
2.1.1.5 0.0045
-
 betaine 37°C, mutant enzym T73G Rattus norvegicus
2.1.1.5 0.0045
-
 L-homocysteine 37°C, mutant enzyme T73G Rattus norvegicus
2.1.1.5 0.0051
-
 L-homocysteine 37°C, mutant enzyme D26A Rattus norvegicus
2.1.1.5 0.0051
-
 betaine 37°C, mutant enzyme D26A Rattus norvegicus
2.1.1.5 0.0058
-
 L-homocysteine 37°C, mutant enzyme T184G Rattus norvegicus
2.1.1.5 0.0058
-
 betaine 37°C, mutant enzyme T184G Rattus norvegicus
2.1.1.5 0.006
-
 L-homocysteine 37°C, mutant enzyme C186S Rattus norvegicus
2.1.1.5 0.006
-
 L-homocysteine 37°C, mutant enzyme E21A Rattus norvegicus
2.1.1.5 0.006
-
 betaine 37°C, mutant enzyme E21A Rattus norvegicus
2.1.1.5 0.006
-
 betaine 37°C, mutant enzyme T186S Rattus norvegicus
2.1.1.5 0.0072
-
 L-homocysteine 37°C, mutant enzyme F74A Rattus norvegicus
2.1.1.5 0.0072
-
 betaine 37°C, mutant enzyme F74A Rattus norvegicus
2.1.1.5 0.0075
-
 L-homocysteine 37°C, mutant enzyme A119G Rattus norvegicus
2.1.1.5 0.0075
-
 betaine 37°C, mutant enzyme A119G Rattus norvegicus
2.1.1.5 0.0076
-
 L-homocysteine 37°C, mutant enzyme C186A Rattus norvegicus
2.1.1.5 0.0076
-
 betaine 37°C, mutant enzyme C186A Rattus norvegicus
2.1.1.5 0.009
-
 L-homocysteine 37°C, mutant enzyme E21K Rattus norvegicus
2.1.1.5 0.009
-
 betaine 37°C, mutant enzyme E21K Rattus norvegicus
2.1.1.5 0.014
-
 L-homocysteine 37°C, wild-type enzyme Rattus norvegicus
2.1.1.5 0.014
-
 betaine 37°C, wild-type enzyme Rattus norvegicus