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Literature summary extracted from

  • Gao, B.; Ellis, H.R.
    Altered mechanism of the alkanesulfonate FMN reductase with the monooxygenase enzyme (2005), Biochem. Biophys. Res. Commun., 331, 1137-1145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.5
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.30 NADP+
-
 Escherichia coli
1.5.1.30 NADPH
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.000016
-
 FMN 25°C, single-enzyme assay Escherichia coli
1.5.1.30 0.00013
-
 FMN 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate Escherichia coli
1.5.1.30 0.0054
-
 NADPH 25°C, single-enzyme assay Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.5 41605
-
 4 * 41605, mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.5 an alkansulfonate + FMNH2 + O2 Escherichia coli
-
 an aldehyde + FMN + sulfite + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Escherichia coli
-
 enzyme SsuE is part of alkanesulfonate monooxygenase system, together with a monooxygenase enzyme SsuD
-
1.14.14.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.5
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.30 reduced riboflavin + NADP+ = riboflavin + NADPH + H+ single-enzyme kinetic assay, SsuE follows ordered sequential mechanism with NADPH as the first substrate to bind and NADP+ as the last product to leave. In presence of monooxygenase SsuD and octanesulfonate mechanism is altered to rapid equilibrium ordered mechanism, and Km-value for FMN increases 10fold Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH
-
 Escherichia coli FMNH2 + NADP+
-
 ?
1.14.14.5 an alkansulfonate + FMNH2 + O2
-
 Escherichia coli an aldehyde + FMN + sulfite + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.5 tetramer 4 * 41605, mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 alkanesulfonate FMN reductase
-
 Escherichia coli
1.5.1.30 SsuE
-
 Escherichia coli
1.14.14.5 SsuD
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.30 1.7
-
 FMN 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate Escherichia coli
1.5.1.30 1.9
-
 FMN 25°C, single-enzyme assay Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN
-
 Escherichia coli
1.5.1.30 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.30 0.0035
-
 NADPH 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate Escherichia coli
1.5.1.30 0.0039
-
 NADPH 25°C, single-enzyme assay Escherichia coli
1.5.1.30 0.0139
-
 NADP+ 25°C, single-enzyme assay Escherichia coli