EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.17 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.17 | D407F | site-directed mutagenesis, mutant shows 8% of wild-type activity | Rattus norvegicus |
1.14.14.17 | K399F | site-directed mutagenesis, mutant shows 28% of wild-type activity | Rattus norvegicus |
1.14.14.17 | K399F/R400F/D407F | site-directed mutagenesis, triple mutant shows 10% of wild-type activity | Rattus norvegicus |
1.14.14.17 | K399P/R400P/D407P | site-directed mutagenesis, triple mutant shows 10% of wild-type activity | Rattus norvegicus |
1.14.14.17 | K399W/R400W/D407W | site-directed mutagenesis, inactive mutant | Rattus norvegicus |
1.14.14.17 | additional information | construction of a truncated enzyme mutant | Rattus norvegicus |
1.14.14.17 | R400F | site-directed mutagenesis, mutant shows 24% of wild-type activity | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.17 | 26-hydroxysqualene | competitive | Rattus norvegicus | |
1.14.14.17 | NB-598 | squalenoid noncompetitive allylamine inhibitor | Rattus norvegicus | |
1.14.14.17 | trisnorsqualene alcohol | squalenoid noncompetitive inhibitor, mechanism-based inactivator | Rattus norvegicus | |
1.14.14.17 | trisnorsqualene alcohol diazoester | i.e. TNSA-Dza, competitive, able to photocovalently modify the native protein | Rattus norvegicus | |
1.14.14.17 | trisnorsqualene cyclopropylamine | squalenoid noncompetitive inhibitor, mechanism-based inactivator | Rattus norvegicus | |
1.14.14.17 | trisnorsqualene hydroxylamine | mechanism-based inactivator | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.17 | 50000 | - |
2 * 50000, recombinant truncated mutant enzyme, SDS-PAGE | Rattus norvegicus |
1.14.14.17 | 100000 | - |
recombinant truncated mutant enzyme | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.17 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.17 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.17 | squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O | active site involves the key residues K399, R400, and D407 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.17 | additional information | location of the substrate-binding site by binding studies using the photolabeling inhibitor trisnorsqualene alcohol diazoester, key residues are K399, R400, and D407 | Rattus norvegicus | ? | - |
? | |
1.14.14.17 | squalene + reduced acceptor + O2 | - |
Rattus norvegicus | (S)-squalene-2,3-epoxide + acceptor + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.17 | dimer | 2 * 50000, recombinant truncated mutant enzyme, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.17 | SE | - |
Rattus norvegicus |
1.14.14.17 | squalene epoxidase | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.17 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.17 | 7.4 | - |
assay at | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.17 | additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
1.14.14.17 | 0.0184 | - |
trisnorsqualene alcohol diazoester | recombinant truncated enzyme mutant, pH 7.4, 37°C | Rattus norvegicus |