Literature summary extracted from

Sengupta, R.; Sahoo, R.; Mukherjee, S.; Regulski, M.; Tully, T.; Stuehr, D.J.; Ghosh, S.
Characterization of Drosophila nitric oxide synthase: a biochemical study (2003), Biochem. Biophys. Res. Commun., 306, 590-597.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.14.13.39	Calmodulin	substrate 2,6-dichlorophenolindophenol, about 10fold increase in activity in presence of calmodulin	Drosophila melanogaster

General Stability

EC Number	General Stability	Organism
1.14.13.39	very stable against trypsin, reductase domain is much more susceptible to urea than oxygenase domain	Drosophila melanogaster

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.13.39	152000	-	x * 152000, SDS-PAGE, plus a proteolytic artefact of 121000	Drosophila melanogaster

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.13.39	Drosophila melanogaster	-	expression in Escherichia coli	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
1.14.13.39	flavoprotein	-	Drosophila melanogaster
1.14.13.39	additional information	hemoprotein	Drosophila melanogaster

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.13.39	2,6-dichlorophenolindophenol + NADPH + O2	best substrate, about 10-fold increase in activity in presence of calmodulin	Drosophila melanogaster	? + NO + NADP+	-	?
1.14.13.39	ferricyanide + NADPH + O2	-	Drosophila melanogaster	ferrocyanide + NADP+ + ?	-	?
1.14.13.39	ferricytochrome c + NADPH + O2	-	Drosophila melanogaster	ferrocytochrome c + NO + NADP+	-	?
1.14.13.39	L-arginine + H2O2 + tetrahydrobiopterin	-	Drosophila melanogaster	? + NO + NADP+	-	?
1.14.13.39	L-arginine + NADPH + O2 + tetrahydrobiopterin	-	Drosophila melanogaster	citrulline + NO + NADP+ + ?	-	?
1.14.13.39	N-hydroxy-L-arginine + H2O2 + tetrahydrobiopterin	-	Drosophila melanogaster	? + NADP+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.13.39	?	x * 152000, SDS-PAGE, plus a proteolytic artefact of 121000	Drosophila melanogaster

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.13.39	DNOS	-	Drosophila melanogaster

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.13.39	5,6,7,8-tetrahydro-L-biopterin	-	Drosophila melanogaster
1.14.13.39	NADPH	-	Drosophila melanogaster

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Release 2023.1 (January 2023)