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Literature summary extracted from

  • Bobst, C.E.; Tabita, F.R.
    The role of cysteine 160 in thiamine diphosphate binding of the Calvin-Benson-Bassham cycle transketolase of Rhodobacter sphaeroides (2004), Arch. Biochem. Biophys., 426, 43-54.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 C160A 81% of wild-type activity Cereibacter sphaeroides
2.2.1.1 C160D 26% of wild-type activity Cereibacter sphaeroides
2.2.1.1 C160E 7% of wild-type activity Cereibacter sphaeroides
2.2.1.1 C160S 59% of wild-type activity Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.0056
-
 D-xylulose 5-phosphate wild-type, pH 6.7, 25°C Cereibacter sphaeroides
2.2.1.1 0.011
-
 D-xylulose 5-phosphate wild-type, pH 7.8, 25°C Cereibacter sphaeroides
2.2.1.1 0.015
-
 D-xylulose 5-phosphate mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
2.2.1.1 0.02
-
 D-xylulose 5-phosphate mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
2.2.1.1 0.033
-
 D-xylulose 5-phosphate mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
2.2.1.1 0.034
-
 D-xylulose 5-phosphate wild-type, pH 8.5, 25°C Cereibacter sphaeroides
2.2.1.1 0.065
-
 D-xylulose 5-phosphate mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
2.2.1.1 0.356
-
 D-ribose 5-phosphate wild-type, pH 6.7, 25°C Cereibacter sphaeroides
2.2.1.1 0.436
-
 D-ribose 5-phosphate mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
2.2.1.1 0.739
-
 D-ribose 5-phosphate mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
2.2.1.1 0.837
-
 D-ribose 5-phosphate mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
2.2.1.1 0.882
-
 D-ribose 5-phosphate mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
2.2.1.1 0.949
-
 D-ribose 5-phosphate wild-type, pH 7.8, 25°C Cereibacter sphaeroides
2.2.1.1 2.316
-
 D-ribose 5-phosphate wild-type, pH 8.5, 25°C Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 69000
-
 2 * 69000, SDS-PAGE Cereibacter sphaeroides
2.2.1.1 122000
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Cereibacter sphaeroides
-
 overexpression in Escherichia coli
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.2.1.1 inactivation in presence of oxygen Cereibacter sphaeroides

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 recombinant protein Cereibacter sphaeroides

Reaction

EC Number Reaction Comment Organism Reaction ID
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate equilibrium binding constants, first order rates of binding of cofactor Cereibacter sphaeroides
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 2.7
-
 mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
2.2.1.1 9.5
-
 mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
2.2.1.1 21.8
-
 mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
2.2.1.1 30.1
-
 mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
2.2.1.1 37
-
 wild-type, pH 7.8, 25°C Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Cereibacter sphaeroides sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.1 dimer 2 * 69000, SDS-PAGE Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 7.6
-
 mutant C160E Cereibacter sphaeroides
2.2.1.1 7.8
-
 wild-type Cereibacter sphaeroides
2.2.1.1 7.8
-
 mutant C160A Cereibacter sphaeroides
2.2.1.1 7.9
-
 mutant C160S Cereibacter sphaeroides
2.2.1.1 8.5
-
 mutant C160D Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate Km-value 0.0018 mM Cereibacter sphaeroides