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Literature summary extracted from

  • Bonner, C.A.; Jensen, R.A.
    Novel features of prephenate aminotransferase from cell cultures of Nicotiana silvestris (1985), Arch. Biochem. Biophys., 238, 237-246.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.79 pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay Nicotiana sylvestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.79 prephenate substrate inhibition above 1 mM, antagonism by L-glutamate Nicotiana sylvestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.79 prephenate + L-glutamate Nicotiana sylvestris important step in biosynthesis of L-tyrosine and L-phenylalanine L-arogenate + 2-oxoglutarate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.79 Nicotiana sylvestris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.79 partially by ion exchange chromatography Nicotiana sylvestris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.79 cell culture stable cell line ANS-1 Nicotiana sylvestris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.79 additional information
-
 substrate specificity Nicotiana sylvestris
2.6.1.79 0.042
-
 crude enzyme extract, substrates prephenate and L-glutamate Nicotiana sylvestris
2.6.1.79 0.073
-
 partially purified enzyme, substrate pyruvate and L-glutamate Nicotiana sylvestris
2.6.1.79 0.077
-
 partially purified enzyme, substrate prephenate and L-aspartate Nicotiana sylvestris
2.6.1.79 0.118
-
 partially purified enzyme, substrate oxaloacetate and L-aspartate Nicotiana sylvestris
2.6.1.79 0.125
-
 partially purified enzyme, substrate oxaloacetate and L-glutamate Nicotiana sylvestris
2.6.1.79 0.156
-
 partially purified enzyme, substrate 2-oxoglutarate and L-aspartate Nicotiana sylvestris
2.6.1.79 0.157
-
 partially purified enzyme, substrate prephenate and L-glutamate Nicotiana sylvestris
2.6.1.79 0.251
-
 partially purified enzyme, substrate 2-oxoglutarate and L-glutamate Nicotiana sylvestris

Storage Stability

EC Number Storage Stability Organism
2.6.1.79 in presence of pyridoxal 5'-phosphate, EDTA, glycerol, stable for at least one month Nicotiana sylvestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.79 2-oxoglutarate + L-aspartate
-
 Nicotiana sylvestris L-glutamate + oxaloacetate
-
 r
2.6.1.79 2-oxoglutarate + L-glutamate
-
 Nicotiana sylvestris L-glutamate + 2-oxoglutarate
-
 r
2.6.1.79 additional information substrate specificity, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and indolepyruvate using cosubstrate L-glutamate, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and pyruvate using cosubstrate L-aspartate Nicotiana sylvestris ?
-
 ?
2.6.1.79 oxaloacetate + L-aspartate
-
 Nicotiana sylvestris L-aspartate + oxaloacetate
-
 r
2.6.1.79 oxaloacetate + L-glutamate
-
 Nicotiana sylvestris L-aspartate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-aspartate
-
 Nicotiana sylvestris L-arogenate + oxaloacetate
-
 r
2.6.1.79 prephenate + L-glutamate important step in biosynthesis of L-tyrosine and L-phenylalanine Nicotiana sylvestris L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate optimal prephenate concentration is 0.5-1.0 mM Nicotiana sylvestris L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 pyruvate + L-glutamate
-
 Nicotiana sylvestris L-alanine + 2-oxoglutarate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.79 AT-C
-
 Nicotiana sylvestris
2.6.1.79 prephenate aminotransferase
-
 Nicotiana sylvestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.79 70
-
-
 Nicotiana sylvestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.79 37 70
-
 Nicotiana sylvestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.79 70
-
 stable, at least for 17 min Nicotiana sylvestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.79 8.25
-
-
 Nicotiana sylvestris

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.79 pyridoxal 5'-phosphate required, stabilizes the enzyme Nicotiana sylvestris