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Literature summary extracted from

  • van Beilen, J.B.; Holtackers, R.; Luscher, D.; Bauer, U.; Witholt, B.; Duetz, W.A.
    Biocatalytic production of perillyl alcohol from limonene by using a novel Mycobacterium sp. cytochrome P450 alkane hydroxylase expressed in Pseudomonas putida (2005), Appl. Environ. Microbiol., 71, 1737-1744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.52 expression in n-octane grown Pseudomonas putida Mycobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.52 soluble
-
 Mycobacterium sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.52 48000
-
 x * 48000, SDS-PAGE Mycobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.52 (-)-(S)-limonene + NADPH + O2 Mycobacterium sp.
-
 (-)-perillyl alcohol + NADP+ + H2O
-
 ?
1.14.14.52 (-)-(S)-limonene + NADPH + O2 Mycobacterium sp. HXN-1500
-
 (-)-perillyl alcohol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.52 Mycobacterium sp.
-
 HXN-1500
-
1.14.14.52 Mycobacterium sp. HXN-1500
-
 HXN-1500
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.52 ammonium sulfate, phenyl Sepharose, Source Q15 Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.52 (-)-(S)-limonene + NADPH + O2
-
 Mycobacterium sp. (-)-perillyl alcohol + NADP+ + H2O
-
 ?
1.14.14.52 (-)-(S)-limonene + NADPH + O2
-
 Mycobacterium sp. HXN-1500 (-)-perillyl alcohol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.52 ? x * 48000, SDS-PAGE Mycobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.52
-
-
 50% loss of activity after 6 h Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.52 7
-
-
 Mycobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.52 5.5 8 almost no activity at pH 5.5 and pH 8.0, respectively Mycobacterium sp.