EC Number | Application | Comment | Organism |
---|---|---|---|
2.6.1.18 | additional information | the enzyme can be utilized as a catalyst for kinetic resolution of beta-amino acids and amines | Achromobacter denitrificans |
2.6.1.18 | synthesis | enzymatic synthesis of enantiomerically pure D-beta-amino-n-butyric acid from racemic beta-amino-n-butyric acid | Achromobacter denitrificans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.18 | gene aptA, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Escherichia coli strain BL21 | Achromobacter denitrificans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.18 | 3-amino-2,3-dihydrobenzoic acid | i.e. gabaculine, strong inhibition | Achromobacter denitrificans | |
2.6.1.18 | aminooxyacetic acid | strong inhibition | Achromobacter denitrificans | |
2.6.1.18 | hydroxylamine | strong inhibition | Achromobacter denitrificans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.18 | additional information | - |
additional information | kinetics | Achromobacter denitrificans | |
2.6.1.18 | 11 | - |
pyruvate | recombinant enzyme | Achromobacter denitrificans | |
2.6.1.18 | 56 | - |
L-beta-amino-n-butyric acid | recombinant enzyme | Achromobacter denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.18 | L-beta-amino-n-butyric acid + pyruvate | Achromobacter denitrificans | - |
3-oxo-n-butyric acid + alanine | - |
? | |
2.6.1.18 | L-beta-amino-n-butyric acid + pyruvate | Achromobacter denitrificans Y2k-2 | - |
3-oxo-n-butyric acid + alanine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.18 | Achromobacter denitrificans | Q7WWK8 | gene aptA | - |
2.6.1.18 | Achromobacter denitrificans Y2k-2 | Q7WWK8 | gene aptA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.18 | recombinant His-tagged enzyme from Escherichia coli strain BL21 | Achromobacter denitrificans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.18 | 77.2 | - |
purified recombinant enzyme | Achromobacter denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.18 | (R,S)-1,2-dimethylpropylamine + pyruvate | 7.5% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 3-methyl-2-oxobutane + alanine | - |
? | |
2.6.1.18 | (R,S)-1,2-dimethylpropylamine + pyruvate | 7.5% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | 3-methyl-2-oxobutane + alanine | - |
? | |
2.6.1.18 | (R,S)-1,5-dimethylhexylamine + pyruvate | 8.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 6-methyl-2-oxoheptane + alanine | - |
? | |
2.6.1.18 | (R,S)-1,5-dimethylhexylamine + pyruvate | 8.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | 6-methyl-2-oxoheptane + alanine | - |
? | |
2.6.1.18 | (R,S)-2-aminoheptane + pyruvate | 9.1% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 2-oxoheptane + alanine | - |
? | |
2.6.1.18 | (R,S)-sec-butylamine + pyruvate | 7.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | ? + alanine | - |
? | |
2.6.1.18 | (S)-alpha-methylbenzylamine + pyruvate | 14% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | acetophenone + alanine | - |
? | |
2.6.1.18 | beta-alanine + pyruvate | 10% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | malonic semialdehyde + L-alanine | - |
? | |
2.6.1.18 | beta-alanine + pyruvate | 10% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | malonic semialdehyde + L-alanine | - |
? | |
2.6.1.18 | butyraldehyde + (S)-alpha-methylbenzylamine | 118% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? | |
2.6.1.18 | glyoxylate + (S)-alpha-methylbenzylamine | 13% of activity with pyruvate | Achromobacter denitrificans | glycine + acetophenone | - |
? | |
2.6.1.18 | L-beta-amino-n-butyric acid + pyruvate | - |
Achromobacter denitrificans | 3-oxo-n-butyric acid + alanine | - |
? | |
2.6.1.18 | L-beta-amino-n-butyric acid + pyruvate | - |
Achromobacter denitrificans Y2k-2 | 3-oxo-n-butyric acid + alanine | - |
? | |
2.6.1.18 | L-beta-amino-n-butyric acid + pyruvate | high activity, highly stereoselective enzyme | Achromobacter denitrificans | 3-oxobutyrate + alanine | - |
? | |
2.6.1.18 | L-beta-homoleucine + pyruvate | 2.6% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 5-methyl-3-oxohexanoate + alanine | - |
? | |
2.6.1.18 | L-beta-leucine + pyruvate | 4.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 4-methyl-3-oxopentanoate + alanine | - |
? | |
2.6.1.18 | additional information | substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates | Achromobacter denitrificans | ? | - |
? | |
2.6.1.18 | additional information | substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates | Achromobacter denitrificans Y2k-2 | ? | - |
? | |
2.6.1.18 | propion aldehyde + (S)-alpha-methylbenzylamine | 105% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? | |
2.6.1.18 | pyruvic aldehyde + (S)-alpha-methylbenzylamine | 5% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.18 | AptA | - |
Achromobacter denitrificans |
2.6.1.18 | omega-amino acid:pyruvate transaminase | - |
Achromobacter denitrificans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.18 | pyridoxal 5'-phosphate | - |
Achromobacter denitrificans |