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Literature summary extracted from

  • Coque, J.J.; Alvarez-Rodriguez, M.L.; Larriba, G.
    Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines (2003), Appl. Environ. Microbiol., 69, 5089-5095.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.136 5-methyltetrahydrofolic acid 1 mM, activation to 119% of control Trichoderma longibrachiatum
2.1.1.136 DTT 1 mM + 1 mM PMSF, activation to 147% of control Trichoderma longibrachiatum
2.1.1.136 glycerol 10%, activation to 122% of control Trichoderma longibrachiatum
2.1.1.136 PMSF 1 mM + 1 mM DTT, activation to 147% of control Trichoderma longibrachiatum

General Stability

EC Number General Stability Organism
2.1.1.136 freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity Trichoderma longibrachiatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.136 Ag+ 1 mM, 94% inhibition Trichoderma longibrachiatum
2.1.1.136 Ca2+ 1 mM, 16% inhibition Trichoderma longibrachiatum
2.1.1.136 Cu2+ 1 mM, 96% inhibition Trichoderma longibrachiatum
2.1.1.136 Hg2+ 0.1 mM, 96% inhibition Trichoderma longibrachiatum
2.1.1.136 S-adenosyl-L-homocysteine competitive Trichoderma longibrachiatum
2.1.1.136 Zn2+ 0.1 mM,82% inhibition Trichoderma longibrachiatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.136 0.1359
-
 2,4,6-Trichlorophenol
-
 Trichoderma longibrachiatum
2.1.1.136 0.284
-
 S-adenosyl-L-methionine
-
 Trichoderma longibrachiatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.136 52500
-
 2 * 52500, SDS-PAGE Trichoderma longibrachiatum
2.1.1.136 112000
-
 gel filtration Trichoderma longibrachiatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine Trichoderma longibrachiatum activity is induced by 2,4,6-trichlorophenol 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine Trichoderma longibrachiatum CECT 20431 activity is induced by 2,4,6-trichlorophenol 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.136 Trichoderma longibrachiatum
-
 CECT 20431
-
2.1.1.136 Trichoderma longibrachiatum CECT 20431
-
 CECT 20431
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.136
-
 Trichoderma longibrachiatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.136 mycelium
-
 Trichoderma longibrachiatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.136 0.00055
-
-
 Trichoderma longibrachiatum

Storage Stability

EC Number Storage Stability Organism
2.1.1.136 -20°C, 10% glycerol, stable for up to 3 months Trichoderma longibrachiatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.136 2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine 10.7% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine 10.7% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3,4-trichlorophenol + S-adenosyl-L-methionine 93.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3,4-trichlorophenol + S-adenosyl-L-methionine 93.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3,6-trichlorophenol + S-adenosyl-L-methionine 55.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3,6-trichlorophenol + S-adenosyl-L-methionine 55.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,3-dichlorophenol + S-adenosyl-L-methionine 22.5% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,5-trichlorophenol + S-adenosyl-L-methionine 64.2% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-tribromophenol + S-adenosyl-L-methionine 13% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine activity is induced by 2,4,6-trichlorophenol Trichoderma longibrachiatum 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine 60% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine activity is induced by 2,4,6-trichlorophenol Trichoderma longibrachiatum CECT 20431 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4,6-trichlorophenol + S-adenosyl-L-methionine 60% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4-dibromophenol + S-adenosyl-L-methionine 36.8% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,4-dichlorophenol + S-adenosyl-L-methionine
-
 Trichoderma longibrachiatum 2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,5-dichlorophenol + S-adenosyl-L-methionine 19.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2,6-dichlorophenol + S-adenosyl-L-methionine 10.4% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 2-chlorophenol + S-adenosyl-L-methionine 9.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2-chloroanisole + S-adenosyl-L-homocysteine
-
 ?
2.1.1.136 3,4-dichlorophenol + S-adenosyl-L-methionine 9.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.136 dimer 2 * 52500, SDS-PAGE Trichoderma longibrachiatum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.136 CPOMT
-
 Trichoderma longibrachiatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.136 28
-
-
 Trichoderma longibrachiatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.136 42
-
 54% loss of activity after 30 min, 89% loss of activity after 1 h Trichoderma longibrachiatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.136 8.2 8.5
-
 Trichoderma longibrachiatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.136 7 8.5 pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum Trichoderma longibrachiatum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.136 0.3789
-
 S-adenosyl-L-homocysteine
-
 Trichoderma longibrachiatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.136 Trichoderma longibrachiatum chromatofocusing pH-range 4-7
-
 4.9