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Literature summary extracted from
- mRNA differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species (2003), Appl. Environ. Microbiol., 69, 334-342.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.22 | expressed as His-tag fusion protein in Escherichia coli | Rhodococcus pyridinivorans |
| 1.14.13.22 | expressed as His-tag fusion protein in Escherichia coli | Arthrobacter sp. |
| 1.14.13.22 | expressed as His-tag fusion protein in Escherichia coli | Rhodococcus ruber |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Rhodococcus ruber | initial step of the degradation of cyclohexanone to adipic acid | epsilon-caprolactone + NADP+ + H2O | - |
? |  |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Rhodococcus pyridinivorans | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Arthrobacter sp. | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Rhodococcus ruber Phi 2 | initial step of the degradation of cyclohexanone to adipic acid | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Rhodococcus pyridinivorans Phi 1 | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | Arthrobacter sp. BP2 | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.22 | Arthrobacter sp. | Q84H88 | - |
- |
| 1.14.13.22 | Arthrobacter sp. BP2 | Q84H88 | - |
- |
| 1.14.13.22 | Rhodococcus pyridinivorans | - |
- |
- |
| 1.14.13.22 | Rhodococcus pyridinivorans Phi 1 | - |
- |
- |
| 1.14.13.22 | Rhodococcus ruber | F5CEP3 | - |
- |
| 1.14.13.22 | Rhodococcus ruber Phi 2 | F5CEP3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.22 | recombinant enzyme using His-tag | Rhodococcus pyridinivorans |
| 1.14.13.22 | recombinant enzyme using His-tag | Arthrobacter sp. |
| 1.14.13.22 | recombinant enzyme using His-tag | Rhodococcus ruber |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 3.57 | - |
with cyclohexanone as substrate, pH 7.5 | Arthrobacter sp. |
| 1.14.13.22 | 3.68 | - |
with cyclohexanone as substrate, pH 7.5 | Rhodococcus pyridinivorans |
| 1.14.13.22 | 3.75 | - |
with cyclohexanone as substrate, pH 7.5 | Rhodococcus ruber |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.22 | cyclohexanone + NADPH + O2 | initial step of the degradation of cyclohexanone to adipic acid | Rhodococcus ruber | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | initial step of the degradation of cyclohexanone to adipic acid | Rhodococcus ruber Phi 2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Rhodococcus pyridinivorans | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Arthrobacter sp. | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Rhodococcus ruber | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Rhodococcus ruber Phi 2 | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Rhodococcus pyridinivorans Phi 1 | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Arthrobacter sp. BP2 | hexano-6-lactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus pyridinivorans | ? | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Arthrobacter sp. | ? | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus ruber | ? | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus ruber Phi 2 | ? | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus pyridinivorans Phi 1 | ? | - |
? | |
| 1.14.13.22 | additional information | also oxidizes a large variety of cyclic and linear ketones | Arthrobacter sp. BP2 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.22 | ChnB | - |
Rhodococcus pyridinivorans |
| 1.14.13.22 | ChnB | - |
Arthrobacter sp. |
| 1.14.13.22 | ChnB | - |
Rhodococcus ruber |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.22 | flavin | - |
Rhodococcus pyridinivorans | |
| 1.14.13.22 | flavin | - |
Arthrobacter sp. | |
| 1.14.13.22 | flavin | - |
Rhodococcus ruber | |
| 1.14.13.22 | NADPH | - |
Rhodococcus pyridinivorans | |
| 1.14.13.22 | NADPH | - |
Arthrobacter sp. | |
| 1.14.13.22 | NADPH | - |
Rhodococcus ruber | |
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