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Literature summary extracted from

  • Erat, M.; Demir, H.; Sakiroglu, H.
    Purification of glutathione reductase from chicken liver and investigation of kinetic properties (2005), Appl. Biochem. Biotechnol., 125, 127-138.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.7 1 mM EDTA is required Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 additional information
-
additional information kinetics Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 43000
-
2 * 43000, SDS-PAGE Gallus gallus
1.8.1.7 100000
-
gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH Gallus gallus
-
glutathione + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 1714fold from liver homogenate, by ammonium sulfate fractionation, affinity chromatography, and gel filtration Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 120
-
purified enzyme from liver Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH
-
Gallus gallus glutathione + NADP+
-
?
1.8.1.7 glutathione disulfide + NADPH optimum ionic strength is 0.75 M using Tris-HCl buffer in presence of EDTA Gallus gallus glutathione + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 43000, SDS-PAGE Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 50
-
-
Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
-
Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.1.7 7.4
-
optimally stable at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
Gallus gallus