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Literature summary extracted from

  • Koon, N.; Squire, C.J.; Baker, E.N.
    Crystallization and preliminary X-ray analysis of alpha-isopropylmalate synthase from Mycobacterium tuberculosis (2004), Acta Crystallogr. Sect. D, 60, 1167-1169.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.13 medicine potential target for drugs against microbial pathogens due to the absence of this enzyme in mammals Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.13 sitting drop vapor diffusion method Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O Mycobacterium tuberculosis first step of leucine biosynthesis alpha-isopropylmalate + CoA
-
 ?
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O Mycobacterium tuberculosis H37Rv first step of leucine biosynthesis alpha-isopropylmalate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis
-
-
-
2.3.3.13 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 recombinant enzyme using His-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O first step of leucine biosynthesis Mycobacterium tuberculosis alpha-isopropylmalate + CoA
-
 ?
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O reaction Mycobacterium tuberculosis alpha-isopropylmalate + CoA
-
 ?
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O first step of leucine biosynthesis Mycobacterium tuberculosis H37Rv alpha-isopropylmalate + CoA
-
 ?
2.3.3.13 acetyl-CoA + alpha-ketoisovalerate + H2O reaction Mycobacterium tuberculosis H37Rv alpha-isopropylmalate + CoA
-
 ?