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Literature summary extracted from

  • Schwarzenbacher, R.; von Delft, F.; Canaves, J.M.; Brinen, L.S.; Dai, X.; Deacon, A.M.; Elsliger, M.A.; Eshaghi, S.; Floyd, R.; Godzik, A.; Grittini, C.; Grzechnik, S.K.; Guda, C.; Jaroszewski, L.; Karlak, C.; Klock, H.E.; Koesema, E.; Kovarik, J.S.; Kreusch, A.; Kuhn, P.; Lesley, S.A.; McMullan, D.; McPhillips, T.M.; Miller, M.A.; Miller, M.D.; Morse, A.; Moy, K.; Ouyang, J.; Page, R.; Robb, A.; Rodrigues, K.; Selby, T.L.; Spraggon, G.; Stevens, R.C.; van den Bedem, H.; Velasquez, J.; Vincent, J.; Wang, X.; West, B.; Wolf, G.; Hodgson, K.O.; Wooley, J.; Wilson, I.A.
    Crystal structure of an iron-containing 1,3-propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 A resolution (2003), Proteins, 54, 174-177.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 gene TM0920, expression of soluble N-terminally MGSDKIHHHHHH-tagged selenomethionine-labeled enzyme in Escherichia coli methionine auxotrophic strain DL41 Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.202 purified recombinant His-tagged protein in 20 mM Tris, pH 7.9, 150 mM NaCl, 0.25 mM TCEP, by nandroplet vapour diffusion method, cyrstallization buffer contains 20% PEG 300, 5% w/v PEG 8000, 10% glycerol, and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 1.3 A resolution, modeling of 2 enzyme molecules, residues 1-359, complexed with 2 molecules of NADP+, 2 ions of Fe2+, 2 molecules of Tris, and 895 water molecules Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.202 Fe2+
-
Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Thermotoga maritima Q9X022 gene TM0920, strain MSB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.202 recombinant tagged selenomethionine-labeled enzyme from Escherichia coli by nickel affinity, ion exchange, and gel filtration chromatography and ultrafiltration Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.202 NAD+
-
Thermotoga maritima