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Literature summary extracted from

  • Hidalgo-Zarco, F.; Camacho, A.G.; Bernier-Villamor, V.; Nord, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Leishmania major (2001), Protein Sci., 10, 1426-1433.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 alpha-beta-imido-dUTP competitive, strong inhibition Leishmania major
3.6.1.23 dUDP competitive Leishmania major
3.6.1.23 dUMP competitive Leishmania major
3.6.1.23 additional information diphosphate has no effect on activity Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0021
-
 dUTP pH 8, 25°C Leishmania major
3.6.1.23 0.0627
-
 dUDP pH 8, 25°C Leishmania major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Co2+ can partially replace Mg2+, causes increase in Km Leishmania major
3.6.1.23 Mg2+ required, no activity in the absence of Mg2+ Leishmania major
3.6.1.23 Mn2+ can partially replace Mg2+, causes increase in Km Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 30300
-
 2 * 30300, calculated from amino acid sequence Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Leishmania major removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dATP + H2O low activity Leishmania major dAMP + diphosphate
-
 ?
3.6.1.23 dCTP + H2O low activity Leishmania major dCMP + diphosphate
-
 ?
3.6.1.23 dGTP + H2O low activity Leishmania major dGMP + diphosphate
-
 ?
3.6.1.23 dTTP + H2O low activity Leishmania major dTMP + diphosphate
-
 ?
3.6.1.23 dUDP + H2O
-
 Leishmania major dUMP + phosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Leishmania major dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival Leishmania major dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 dimer 2 * 30300, calculated from amino acid sequence Leishmania major

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 dUTPase-dUDPase hydrolyzes dUTP and dUDP Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 50
-
-
 Leishmania major

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.23 4 60 very low activity above 60°C Leishmania major

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 4 55 stable against denaturation Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 5.4
-
 dATP pH 8, 25°C Leishmania major
3.6.1.23 6.4
-
 dGTP pH 8, 25°C Leishmania major
3.6.1.23 8.6
-
 dTTP pH 8, 25°C Leishmania major
3.6.1.23 13
-
 dCTP pH 8, 25°C Leishmania major
3.6.1.23 49
-
 dUTP pH 8, 25°C Leishmania major
3.6.1.23 65
-
 dUDP pH 8, 25°C Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 8 10
-
 Leishmania major

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.23 7 10 strong increase of activity between pH 7 and 8 Leishmania major

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.23 0.0009
-
 alpha-beta-imido-dUTP pH 8, 25°C Leishmania major