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Literature summary extracted from

  • Ko, J.H.; Hahm, M.S.; Kang, H.A.; Nam, S.W.; Chung, B.H.
    Secretory expression and purification of Aspergillus niger glucose oxidase in Saccharomyces cerevisiae mutant deficient in PMR1 gene (2002), Protein Expr. Purif., 25, 488-493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.4 expression in Saccharomyces cerevisiae mutant deficient in PMR1 gene. GOD-His6 expressed in wild-type strain is hyperglycosylated, GOD-His6 expressed in pmr1DELTA strain is not hyperglycosylated Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.4 glycoprotein GOD-His6 expressed in wild-type strain of Saccharomyces cerevisiae is hyperglycosylated, GOD-His6 expressed in pmr1DELTA strain of Saccharomyces cerevisiae is not hyperglycosylated. The catalytic efficiency and physical properties of pmr1DELTA mutant-derived GOD-His6 are very similar to those of natural GOD Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4
-
Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 189
-
GOD-His6 expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger
1.1.3.4 194
-
GOD-His6 expressed in pmr1DELTA mutant cells of Saccharomyces cerevisiae Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.4 70
-
pH 5.1, 75% loss of of activity of natural GOD and pmr1DELTA-mutant-derived GOD, 65% loss of activity of hyperglycosylated GOD Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 6
-
natural GOD, pmr1DELTA-mutant-derived GOD,hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.4 4.5 10 pH 4.5: about 85% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae, pH 10: about 75% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger