EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.10 | - |
Pseudomonas sp. |
4.1.3.39 | purified selenomethionine-labeled enzyme complex of 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), protein solution contains 9 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 1 mM DTT, and 2 mM NAD+, mixing of equal volumes of 0.001 ml of protein and reservoir solutions, the latter contains 18% PEG 8000, 100 mM ammonium sulfate, and 100 mM PIPES, pH 7.5, 3 days, crystals are used for microseeding, cryoprotection by soaking in mother liquor with 20% v/v 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 1.7 A resolution | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.10 | iodoacetate | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.10 | 140000 | - |
- |
Pseudomonas sp. |
4.1.3.39 | 32500 | - |
1 * 32500 + 1 * 37500, active unit is an enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE and crystal structure | Pseudomonas sp. |
4.1.3.39 | 37500 | - |
1 * 32500 + 1 * 37500, active unit is an enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE and crystal structure | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.10 | acetaldehyde + CoA + NAD+ | Pseudomonas sp. | involved in degradation of toxic aromatic compounds via the intermediate catechol | acetyl-CoA + NADH | - |
r | |
1.2.1.10 | acetaldehyde + CoA + NAD+ | Pseudomonas sp. CF 600 | involved in degradation of toxic aromatic compounds via the intermediate catechol | acetyl-CoA + NADH | - |
r | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | Pseudomonas sp. | last but one step in meta-cleavage pathway for catechol degradation | pyruvate + acetaldehyde | - |
? | |
4.1.3.39 | additional information | Pseudomonas sp. | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | ? | - |
? | |
4.1.3.39 | additional information | Pseudomonas sp. CF 600 | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.10 | Pseudomonas sp. | - |
- |
- |
1.2.1.10 | Pseudomonas sp. CF 600 | - |
- |
- |
4.1.3.39 | Pseudomonas sp. | - |
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10 | - |
4.1.3.39 | Pseudomonas sp. CF 600 | - |
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.1.10 | acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+ | part of a bifunctional enzyme complex that also displays 4-hydroxy-2-ketovalerate aldolase activity | Pseudomonas sp. | |
4.1.3.39 | (S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate | active site structure, molecular channeling of substrate and intermediate | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.10 | acetaldehyde + CoA + NAD+ | - |
Pseudomonas sp. | acetyl-CoA + NADH | - |
r | |
1.2.1.10 | acetaldehyde + CoA + NAD+ | involved in degradation of toxic aromatic compounds via the intermediate catechol | Pseudomonas sp. | acetyl-CoA + NADH | - |
r | |
1.2.1.10 | acetaldehyde + CoA + NAD+ | - |
Pseudomonas sp. CF 600 | acetyl-CoA + NADH | - |
r | |
1.2.1.10 | acetaldehyde + CoA + NAD+ | involved in degradation of toxic aromatic compounds via the intermediate catechol | Pseudomonas sp. CF 600 | acetyl-CoA + NADH | - |
r | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | - |
Pseudomonas sp. | pyruvate + acetaldehyde | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | last but one step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. | pyruvate + acetaldehyde | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | - |
Pseudomonas sp. CF 600 | pyruvate + acetaldehyde | - |
? | |
4.1.3.39 | additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. | ? | - |
? | |
4.1.3.39 | additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, molecular channeling of substrate and intermediate | Pseudomonas sp. | ? | - |
? | |
4.1.3.39 | additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. CF 600 | ? | - |
? | |
4.1.3.39 | additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, molecular channeling of substrate and intermediate | Pseudomonas sp. CF 600 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.10 | tetramer | composed of two dimers, one dimer accounts for 4-hydroxy-2-ketovalerate aldolase, subunit size 37500, and one for acylating acetaldehyde dehydrogenase, subunit size 32500 | Pseudomonas sp. |
4.1.3.39 | dimer | 1 * 32500 + 1 * 37500, active unit is an enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE and crystal structure | Pseudomonas sp. |
4.1.3.39 | More | enzyme complex structure analysis | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.10 | 4-hydroxy-2-ketovalerate aldolase/acylating acetaldehyde dehydrogenase | bifunctional enzyme | Pseudomonas sp. |
4.1.3.39 | DmpFG | - |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.10 | NAD+ | - |
Pseudomonas sp. |