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Literature summary extracted from

  • Taylor, M.A.; Ross, H.A.; McRae, D.; Wright, F.; Viola, R.; Davies, H.V.
    Copy-DNA cloning and characterisation of a potato alpha-glucosidase: expression in Escherichia coli and effects of down-regulation in transgenic potato (2001), Planta, 213, 258-264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene MAL2, genomic library construction, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli as N- and C-terminally His-tagged protein, expression pattern analysis of the gene in potato plants by Northern blot analysis Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.20 additional information construction of transgenic potato plants, transformation of the antisense construct via Agrobacterium tumefaciens infection, gene is cloned under the control of CaMV S35 promotor, the gene is down-regulated in transgenic plants showing no obvious phenotype, but showing reduced content of sucrose and increased content of fructose and glucose in tubers Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 3.7
-
 maltotetraose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 4.7
-
 maltopentaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 6.9
-
 maltotriose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 8.1
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 15.1
-
 maltoheptaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 33
-
 maltohexaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 34.5
-
 maltose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 additional information Solanum tuberosum phylogenetic analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Solanum tuberosum
-
 gene MAL2w, enzyme MAL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 leaf
-
 Solanum tuberosum
-
3.2.1.20 additional information expression pattern analysis Solanum tuberosum
-
3.2.1.20 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Solanum tuberosum 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Solanum tuberosum maltohexaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Solanum tuberosum maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Solanum tuberosum D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Solanum tuberosum 2 D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Solanum tuberosum maltotriose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Solanum tuberosum maltose + D-glucose
-
 ?
3.2.1.20 additional information phylogenetic analysis Solanum tuberosum ?
-
 ?
3.2.1.20 additional information no activity with xyloglucan oligosaccharides, 4-nitrophenyl alpha-D-xylopyranoside, and gelatinised potato starch Solanum tuberosum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 MAL2
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 30
-
 assay at Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 35.2
-
 maltoheptaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 35.9
-
 maltose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 57.2
-
 maltohexaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 64.3
-
 maltopentaose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 69.6
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 74.6
-
 maltotetraose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum
3.2.1.20 100.4
-
 maltotriose pH 6.8, 30°C, recombinant enzyme Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6.8
-
 assay at Solanum tuberosum