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Literature summary extracted from

  • de la Fuente van Bentem, S.; Vossen, J.H.; Vermeer, J.E.; de Vroomen, M.J.; Gadella, T.W., Jr.; Haring, M.A.; Cornelissen, B.J.
    The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing (2003), Plant Physiol., 133, 702-712.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.16 arachidonic acid
-
 Solanum lycopersicum
3.1.3.16 linoleic acid
-
 Solanum lycopersicum
3.1.3.16 linolenic acid
-
 Solanum lycopersicum
3.1.3.16 oleic acid
-
 Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression in Escherichia coli Rattus norvegicus
3.1.3.16 expression in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.16 additional information removal of the TPR domain leads to a higher specific activity than wild-type enzyme at pH 7.5 but not at pH 5 Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 okadaic acid IC50: 0.0081 mM Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.16 cytoplasm the 55000 Da isoform Solanum lycopersicum 5737
-
3.1.3.16 endoplasmic reticulum the 63000 Da isoform Solanum lycopersicum 5783
-
3.1.3.16 membrane the 63000 Da isoform, integral membran protein Solanum lycopersicum 16020
-
3.1.3.16 nucleus the 55000 Da isoform Solanum lycopersicum 5634
-
3.1.3.16 soluble the 55000 Da isoform Solanum lycopersicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Mg2+ when p-nitrophenyl phosphate is used as substrate 10 mM MgCl2 increases the activity, when phosphocasein is used as substrate 10 mM MgCl2 reduces the activity by 50% Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 55000
-
-
 Solanum lycopersicum
3.1.3.16 63000
-
-
 Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Rattus norvegicus P53042
-
-
3.1.3.16 Solanum lycopersicum Q84K11 tomato
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 0.033
-
 at pH 7.5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum
3.1.3.16 0.034
-
 at pH 5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum
3.1.3.16 0.064
-
 at pH 5 with p-nitrophenyl phosphate as substrate Rattus norvegicus
3.1.3.16 0.081
-
 at pH 7.5 with p-nitrophenyl phosphate as substrate Rattus norvegicus
3.1.3.16 0.245
-
 DELTATPR at pH 7.5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus 4-nitrophenol + phosphate
-
 ?
3.1.3.16 p-nitrophenyl phosphate + H2O
-
 Solanum lycopersicum 4-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphorylated casein + H2O
-
 Rattus norvegicus casein + phosphate
-
 ?
3.1.3.16 phosphorylated casein + H2O
-
 Solanum lycopersicum casein + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 LePP5
-
 Solanum lycopersicum
3.1.3.16 PP5
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.16 0.0081
-
 IC50: 0.0081 mM Solanum lycopersicum okadaic acid