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Literature summary extracted from

  • Sibout, R.; Eudes, A.; Pollet, B.; Goujon, T.; Mila, I.; Granier, F.; Seguin, A.; Lapierre, C.; Jouanin, L.
    Expression pattern of two paralogs encoding cinnamyl alcohol dehydrogenases in Arabidopsis. Isolation and characterization of the corresponding mutants (2003), Plant Physiol., 132, 848-860.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D, DNA and amino acid sequence determination and analysis, tissue expression pattern study of wild-type and mutant plants Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.195 additional information construction of null-mutants of both genes AtCAD-C and AtCAD-D showing highly reduced enzyme activity, but only the latter shows slightly modificated lignin structure, construction of transgenic plants by infection with recombinant Agrobacterium tumefaciens strain C58pMP90 via flower infiltration method, expression pattern of AtCAD-C and AtCAD-D Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.195 42000
-
 x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE Arabidopsis thaliana
1.1.1.195 44000
-
 x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 coniferol + NADP+ Arabidopsis thaliana
-
 coniferyl aldehyde + NADPH
-
 ?
1.1.1.195 additional information Arabidopsis thaliana role of the enzyme in lignin biosynthesis and structure formation ?
-
 ?
1.1.1.195 sinapyl alcohol + NADP+ Arabidopsis thaliana
-
 sinapyl aldehyde + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Arabidopsis thaliana Q02971 9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 flower
-
 Arabidopsis thaliana
-
1.1.1.195 leaf
-
 Arabidopsis thaliana
-
1.1.1.195 additional information enzyme expression pattern of AtCAD-C and AtCAD-D, no expression in silique Arabidopsis thaliana
-
1.1.1.195 root
-
 Arabidopsis thaliana
-
1.1.1.195 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 coniferol + NADP+
-
 Arabidopsis thaliana coniferyl aldehyde + NADPH
-
 ?
1.1.1.195 additional information role of the enzyme in lignin biosynthesis and structure formation Arabidopsis thaliana ?
-
 ?
1.1.1.195 sinapyl alcohol + NADP+
-
 Arabidopsis thaliana sinapyl aldehyde + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.195 ? x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Arabidopsis thaliana
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.195 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.195 8.8
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADPH
-
 Arabidopsis thaliana