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Literature summary extracted from

  • de Kraker, J.W.; Franssen, M.C.R.; Joerink, M.; de Groot, A.; Bouwmeester, H.J.
    Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demostration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory (2002), Plant Physiol., 129, 257-268.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.31 argon 34% activation Cichorium intybus
1.3.1.31 clotrimazole slight activation Cichorium intybus
1.3.1.31 CO atmosphere of 80% v/v with 20% v/v O2, 40% activation Cichorium intybus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.31 aminobenzotriazole slight inhibition Cichorium intybus
1.3.1.31 Metyrapone slight inhibition Cichorium intybus
1.14.14.150 CO the inhibition in the presence of 90% (v/v) CO and 10% (v/v) O2 is reversed to some extent by irradiation with blue light (450 nm) Cichorium intybus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.31 (+)-costunolide + NADPH Cichorium intybus part of the metabolization of (+)-costunolide to leucodin, overview 11(S),13-dihydrocostunolide + NADP+
-
 ?
1.3.1.31 additional information Cichorium intybus enzyme may be part of the O2-dependent cytochrome P450-enzyme (+)-costunolide synthase, pathway, overview ?
-
 ?
1.14.14.150 additional information Cichorium intybus (+)-costunolide synthase is a cytochrome P450 enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Cichorium intybus
-
 chicory
-
1.14.14.150 Cichorium intybus
-
 cultivar Focus
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.31 root
-
 Cichorium intybus
-
1.14.14.150 root
-
 Cichorium intybus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (+)-costunolide + NADPH
-
 Cichorium intybus 11(S),13-dihydrocostunolide + NADP+
-
 ?
1.3.1.31 (+)-costunolide + NADPH part of the metabolization of (+)-costunolide to leucodin, overview Cichorium intybus 11(S),13-dihydrocostunolide + NADP+
-
 ?
1.3.1.31 additional information enzyme may be part of the O2-dependent cytochrome P450-enzyme (+)-costunolide synthase, pathway, overview Cichorium intybus ?
-
 ?
1.14.14.150 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
 Cichorium intybus (+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O (+)-costunolide is detected as its Cope rearrangement product dehydrosaussurea lactone ir
1.14.14.150 additional information (+)-costunolide synthase is a cytochrome P450 enzyme Cichorium intybus ?
-
 ?
1.14.14.150 additional information (+)-costunolide synthase is not capable of converting alpha-costic acid and elema-1,3,11(13)-trien-12-oic acid Cichorium intybus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.31 enoate reductase
-
 Cichorium intybus
1.14.14.150 (+)-costunolide synthase
-
 Cichorium intybus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADPH dependent on, poor activity with NADH Cichorium intybus