Literature summary extracted from

Tausta, S.L.; Coyle, H.M.; Rothermel, B.; Stiefel, V.; Nelson, T.
Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor (2002), Plant Mol. Biol., 50, 635-652.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.40	isozyme DNA and amino acid sequence determination and analysis, phylogenetic analysis, overview	Zea mays

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.40	chloroplast	-	Zea mays	9507	-
1.1.1.40	cytosol	-	Zea mays	5829	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.40	62000	-	x * 62000, isozyme ZmChlMe1, x * 72000, isozyme ZmChlMe2	Zea mays
1.1.1.40	72000	-	x * 62000, isozyme ZmChlMe1, x * 72000, isozyme ZmChlMe2	Zea mays

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.40	Zea mays	Q8W000	isozyme ZmChlMe2; C4 plant, 2 isozymes, a C3-like isozyme, ZmChlMe2, and a C4-like isozyme, ZmChlMe1, most abundant form is the C4-like chloroplastic leaf isozyme	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.40	embryo	-	Zea mays	-
1.1.1.40	endosperm	-	Zea mays	-
1.1.1.40	leaf	young and mature green leaves, in sheath, tass, tip, and husk of the latter	Zea mays	-
1.1.1.40	root	only isozyme ZmChlMe2	Zea mays	-
1.1.1.40	seedling	-	Zea mays	-

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.40	?	x * 62000, isozyme ZmChlMe1, x * 72000, isozyme ZmChlMe2	Zea mays

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.40	NADP-dependent malic enzyme	-	Zea mays
1.1.1.40	NADP-ME	-	Zea mays

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.40	6.9	7.8	isozyme ZmChlMe2	Zea mays
1.1.1.40	8	-	approximately, isozyme ZmChlMe1	Zea mays

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.1.40	Zea mays	isozyme ZmChlMe2	-	4.9
1.1.1.40	Zea mays	isozyme ZmChlMe1	-	6

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Release 2023.1 (January 2023)