EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.12 | functional expression in Pichia pastoris | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | N6-dimethylallyladenine + FAD + H2O | Zea mays | - |
adenine + 3-methylbut-2-enal + FADH2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.12 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.12 | partially, recombinant enzyme from Pichia pastoris | Zea mays |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.99.12 | additional information | - |
assay development and optimization with 2,6-dichlorophenolindophenol as electron acceptor | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | N6-dimethylallyladenine + 2,6-dichlorophenolindophenol | i.e. isopentenyladenine, reaction via an imine intermediate | Zea mays | adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.12 | N6-dimethylallyladenine + FAD + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
? | |
1.5.99.12 | N6-dimethylallyladenine + FAD + H2O2 | i.e. isopentenyladenine | Zea mays | adenine + 3-methylbut-2-enal + FADH2 + O2 | - |
? | |
1.5.99.12 | zeatin + 2,6-dichlorophenolindophenol | - |
Zea mays | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.12 | cytokinin oxidase/dehydrogenase | - |
Zea mays |
1.5.99.12 | ZmCKX1 | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.99.12 | 22 | - |
assay at room temperature | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.12 | 7 | - |
assay at | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | 2,6-dichlorophenolindophenol | in vitro 4000fold faster reaction than with the native acceptor | Zea mays | |
1.5.99.12 | FAD | - |
Zea mays |