Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Laskey, J.G.; Patterson, P.; Bilyeu, K.; Morris, R.O.
    Rate enhancement of cytokinin oxidase/dehydrogenase using 2,6-dichlorophenolindophenol as an electron acceptor (2003), Plant Growth Regul., 40, 189-196.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 functional expression in Pichia pastoris Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 N6-dimethylallyladenine + FAD + H2O Zea mays
-
adenine + 3-methylbut-2-enal + FADH2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.12 partially, recombinant enzyme from Pichia pastoris Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.12 additional information
-
assay development and optimization with 2,6-dichlorophenolindophenol as electron acceptor Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 N6-dimethylallyladenine + 2,6-dichlorophenolindophenol i.e. isopentenyladenine, reaction via an imine intermediate Zea mays adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.12 N6-dimethylallyladenine + FAD + H2O
-
Zea mays adenine + 3-methylbut-2-enal + FADH2
-
?
1.5.99.12 N6-dimethylallyladenine + FAD + H2O2 i.e. isopentenyladenine Zea mays adenine + 3-methylbut-2-enal + FADH2 + O2
-
?
1.5.99.12 zeatin + 2,6-dichlorophenolindophenol
-
Zea mays ? + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 cytokinin oxidase/dehydrogenase
-
Zea mays
1.5.99.12 ZmCKX1
-
Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.12 22
-
assay at room temperature Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.12 7
-
assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 2,6-dichlorophenolindophenol in vitro 4000fold faster reaction than with the native acceptor Zea mays
1.5.99.12 FAD
-
Zea mays