Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, L.M.; Omiya, T.; Hata, S.; Izui, K.
    Molecular characterization of a phosphoenolpyruvate carboxylase from a thermophilic cyanobacterium, Synechococcus vulcanus with unusual allosteric properties (2002), Plant Cell Physiol., 43, 159-169.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 3-phosphoglycerate slight stimulation Thermostichus vulcanus
4.1.1.31 fructose 1,6-diphosphate activates Thermostichus vulcanus
4.1.1.31 Gln slight stimulation Thermostichus vulcanus
4.1.1.31 Glu slight stimulation Thermostichus vulcanus
4.1.1.31 glucose 1-phosphate slight stimulation Thermostichus vulcanus
4.1.1.31 glucose 6-phosphate slight stimulation Thermostichus vulcanus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.31 expression in Escherichia coli Thermostichus vulcanus

General Stability

EC Number General Stability Organism
4.1.1.31 retains full activity after exposure to 0.5 M guanidine-HCl at 30°C for 20 h Thermostichus vulcanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 alpha-ketoglutarate 10 mM, 9% inhibition Thermostichus vulcanus
4.1.1.31 ATP 5.0 mM, 57% inhibition Thermostichus vulcanus
4.1.1.31 D-fructose 10 mM, 19% inhibition Thermostichus vulcanus
4.1.1.31 D-Fructose 1-phosphate 10 mM, 26% inhibition Thermostichus vulcanus
4.1.1.31 D-fructose 2,6-diphosphate 0.3 mM, 35% inhibition Thermostichus vulcanus
4.1.1.31 fumarate 10 mM, 31% inhibition Thermostichus vulcanus
4.1.1.31 L-Asp more sensitive to inhibition by Asp at pH 9.0 than at pH 7.0 Thermostichus vulcanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 120000
-
 4 * 120000, SDS-PAGE Thermostichus vulcanus
4.1.1.31 570000
-
 gel filtration Thermostichus vulcanus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Thermostichus vulcanus P0A3X6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Thermostichus vulcanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Thermostichus vulcanus phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 120000, SDS-PAGE Thermostichus vulcanus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 SvPEPC
-
 Thermostichus vulcanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 42
-
 at pH 7.5 Thermostichus vulcanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.31 30 50 30°C: about 40% of maximal activity, 50°C: about 55% of maximal activity Thermostichus vulcanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 50
-
 60 min, stable Thermostichus vulcanus
4.1.1.31 60
-
 10 min, 50% loss of activity Thermostichus vulcanus
4.1.1.31 70
-
 20 min, complete inactivation Thermostichus vulcanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 9
-
 at 30°C Thermostichus vulcanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 7 9.5 pH 7.0: about 65% of maximal activity, pH 9.5: about 80% of maximal activity Thermostichus vulcanus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.31 0.025
-
 Asp pH 9.0 Thermostichus vulcanus
4.1.1.31 2
-
 Asp pH 7.5 Thermostichus vulcanus