EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.2 | Cu2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum | |
3.2.1.2 | Hg2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum | |
3.2.1.2 | Mn2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum | |
3.2.1.2 | p-chloromercuribenzoate | 0.01 mM, 100% inhibition | Panicum miliaceum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 58000 | - |
gel filtration | Panicum miliaceum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.2 | starch + H2O | Panicum miliaceum | beta-amylase should be a key enzyme in starch degradation during the germination of millet seeds, enzyme activity increases during days 1-4 of germination | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.2 | Panicum miliaceum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.2 | - |
Panicum miliaceum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.2 | seed | germinating | Panicum miliaceum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 103.9 | - |
pH 4.5, 37°C, soluble starch | Panicum miliaceum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.2 | amylopectin + H2O | 114.6% of the activity with amylose, soluble starch, amylose and amylopectin are the most suitable substrates, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | amylose + H2O | EX-I, soluble starch, amylose and amylopectin are the most suitable substrates, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | glycogen + H2O | 49.4% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltoheptaose + H2O | 34.6% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltohexaose + H2O | 23.3% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltooligosaccharide + H2O | beta-amylase hydrolyzes maltooligosaccharides more readily as their degree of polymerization increases, this being strongest for maltooligosaccharides larger than 13 glucose residues and very weakly for maltotriose, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltopentaose + H2O | 13.8% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltotetraose + H2O | 7.6% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | maltotriose + H2O | very poor substrate | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | starch + H2O | beta-amylase should be a key enzyme in starch degradation during the germination of millet seeds, enzyme activity increases during days 1-4 of germination | Panicum miliaceum | ? | - |
? | |
3.2.1.2 | starch + H2O | 106.9% of the activity with amylose, soluble starch, amylose and amylopectin are the most suitable substrates, some activity against native starch, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.2 | monomer | 1 * 58000, SDS-PAGE | Panicum miliaceum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 55 | - |
optimum temperature after 30 min incubation | Panicum miliaceum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 55 | - |
15 min, 50 mM NaOAc buffer, pH 6, stable up to | Panicum miliaceum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 5.5 | 6 | - |
Panicum miliaceum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.2 | 3.5 | 9 | 15 h, 20°C, 50 mM McIlvaines buffer and sodium carbonate-boric acid buffer, stable in the range | Panicum miliaceum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.2 | Panicum miliaceum | isoelectric focusing | - |
4.9 |