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Literature summary extracted from

  • Tripodi, K.E.J.; Gomez Casati, M.E.; Uttaro, A.D.; Podesta, F.E.
    Expression and characterization of recombinant cytosolic NAD+-dependent malate dehydrogenase from Mesembryanthemum crystallinum (2003), Physiol. Plant., 117, 222-228.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 expression in Escherichia coli Mesembryanthemum crystallinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 oxaloacetate
-
 Mesembryanthemum crystallinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0088
-
 oxaloacetate pH 6.8, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.0136
-
 NADH pH 8.2, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.0341
-
 L-malate pH 8.2, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.0366
-
 NADH pH 6.8, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.0449
-
 oxaloacetate pH 8.2, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.319
-
 NAD+ pH 8.2, 30°C Mesembryanthemum crystallinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Mesembryanthemum crystallinum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 39500
-
 x * 39500, SDS-PAGE Mesembryanthemum crystallinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 additional information Mesembryanthemum crystallinum the enzyme plays a central role in Crassulacean acid metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Mesembryanthemum crystallinum O24047
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 leaf
-
 Mesembryanthemum crystallinum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 2-oxoglutarate + NADH + H+ about 10% of the activity with oxaloacetate at pH 6.8 and at pH 8.2 Mesembryanthemum crystallinum 2-hydroxyglutarate + NAD+
-
 ?
1.1.1.37 3-bromopyruvate + NADH about 10% of the activity with oxaloacetate at pH 6.8 Mesembryanthemum crystallinum 3-bromo-2-hydroxypropanoate + NAD+
-
 ?
1.1.1.37 alpha-ketoisovalerate + NADH about 10% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxyisovalerate + NAD+
-
 ?
1.1.1.37 additional information the enzyme plays a central role in Crassulacean acid metabolism Mesembryanthemum crystallinum ?
-
 ?
1.1.1.37 additional information less than 10% of the activity with oxaloacetate with the substrates: alpha-ketobutyrate, alpha-ketoisocaproate, alpha-ketovalerate and alpha-ketomethylvalerate Mesembryanthemum crystallinum ?
-
 ?
1.1.1.37 oxaloacetate + NADH the ratio of oxaloacetate reduction to malate oxidation is 28.4 Mesembryanthemum crystallinum L-malate + NAD+
-
 r
1.1.1.37 phenylpyruvate + NADH about 30% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
1.1.1.37 pyruvate + NADH about 25% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxypropanoate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 ? x * 39500, SDS-PAGE Mesembryanthemum crystallinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 50
-
 reduction of oxaloacetate Mesembryanthemum crystallinum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.37 30 58 30°C: about 50% of maximal activity, 58°C: about 55% of maximal activity Mesembryanthemum crystallinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 50
-
 5 min, stable up to Mesembryanthemum crystallinum
1.1.1.37 60
-
 5 min, about 80% loss of activity Mesembryanthemum crystallinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.5 9.5 reduction of oxaloacetate Mesembryanthemum crystallinum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Mesembryanthemum crystallinum
1.1.1.37 NADH
-
 Mesembryanthemum crystallinum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.37 0.225
-
 oxaloacetate pH 6.8, 30°C Mesembryanthemum crystallinum
1.1.1.37 0.775
-
 oxaloacetate pH 8.2, 30°C Mesembryanthemum crystallinum