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Literature summary extracted from

  • Vasquez, D.A.; Nyaga, S.G.; Lloyd, R.S.
    Purification and characterization of a novel UV lesion-specific DNA glycosylase/AP lyase from Bacillus sphaericus (2000), Mutat. Res., 459, 307-316.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.17 NaBH4 reduces the imino intermediate resulting in a stable covalent linkage between the enzyme and the DNA Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.17 26000
-
 x * 26000, SDS-PAGE and gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.17 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.17 to homogeinity, using several chromatographic steps Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.17 additional information unable to incise DNA containing either A:G, A:8-oxoG or 8-oxoG:C mismatches Lysinibacillus sphaericus ?
-
 ?
3.2.2.17 oligonucleotide + H2O 49-mers containing a single site-specific cis-syn, trans-syn I or trans-syn II thymine dimer Lysinibacillus sphaericus ? beta elimination reaction results in products wich have 3'4-hydroxy-2-pentanal ends ?
3.2.2.17 oligonucleotide + H2O 49-mers radiolabeled containing a site-specific uracil with uracil DNA glycosylase Lysinibacillus sphaericus ? incise of AP sites resulting in beta- and delta-elimination products with 3'4-hydroxy-2-pentenal and 3'-phosphate termini respectively ?
3.2.2.17 oligonucleotide + H2O containing either a (6-4) or Dewar photoproduct of TpT Lysinibacillus sphaericus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.17 ? x * 26000, SDS-PAGE and gel filtration Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
3.2.2.17 Bsp-pdg
-
 Lysinibacillus sphaericus