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Literature summary extracted from

  • Abel, S.; Kock, M.
    Secretory acid ribonucleases from tomato, Lycopersicon esculentum Mill (2001), Methods Enzymol., 341, 351-368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.19 citrate 25 mM sodium citrate buffer results in 170% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 Solanum lycopersicum
4.6.1.19 additional information enzymes are induced by phosphate starvation Solanum lycopersicum
4.6.1.19 phosphate 25 mM sodium phosphate buffer results in 153% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.19
-
Arabidopsis thaliana
4.6.1.19
-
Zinnia elegans
4.6.1.19 enzymes are S-gene products, cloning of RNases Le and LX, DNA and amino acid sequence determination and analysis Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.19 4-chloromercuribenzoic acid 80% inhibition of RNase LE at 1 mM Solanum lycopersicum
4.6.1.19 Ag+ complete inhibition at 10 mM Solanum lycopersicum
4.6.1.19 Al3+ complete inhibition at 10 mM Solanum lycopersicum
4.6.1.19 Cu2+ complete inhibition at 10 mM Solanum lycopersicum
4.6.1.19 Hg2+ complete inhibition at 10 mM Solanum lycopersicum
4.6.1.19 additional information no effect of 2.5 mM EDTA, no product inhibition Solanum lycopersicum
4.6.1.19 Zn2+ complete inhibition at 10 mM Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.19 endoplasmic reticulum RNase LX Solanum lycopersicum 5783
-
4.6.1.19 extracellular RNase LE Solanum lycopersicum
-
-
4.6.1.19 microsome RNase LX Solanum lycopersicum
-
-
4.6.1.19 vacuole RNases LV1-3 Solanum lycopersicum 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.19 additional information RNases LE, LX and LV-3 do not require divalent cations Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.19 additional information Solanum lycopersicum besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions ?
-
?
4.6.1.19 additional information Arabidopsis thaliana enzyme plays a role in self-incompatibility cell-to-cell interactions ?
-
?
4.6.1.19 additional information Zinnia elegans enzyme plays a role in self-incompatibility cell-to-cell interactions ?
-
?
4.6.1.19 additional information Momordica charantia enzyme plays a role in self-incompatibility cell-to-cell interactions ?
-
?
4.6.1.19 additional information Nicotiana glutinosa enzyme plays a role in self-incompatibility cell-to-cell interactions ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Arabidopsis thaliana
-
-
-
4.6.1.19 Momordica charantia
-
bitter gourd, RNase MC1
-
4.6.1.19 Nicotiana glutinosa Q9SSV1 RNase NGR3
-
4.6.1.19 Solanum lycopersicum
-
1 extracellular, 3 vacuolar and 1 microsomal enzyme forms
-
4.6.1.19 Zinnia elegans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.6.1.19 additional information the enzymes contain secretory targeting signals, RNase LX contains an ER retention signal sequence Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.19
-
Momordica charantia
4.6.1.19
-
Nicotiana glutinosa
4.6.1.19 preparation of microsomes, protoplasts, vacuoles, cell culture supernatant, purification of RNase LE, LX, and LV-1-3, different methods, 1800-4400fold, overview Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.19 cell suspension culture phosphate-starved Solanum lycopersicum
-
4.6.1.19 culture medium from phosphate-starved cells Solanum lycopersicum
-
4.6.1.19 leaf
-
Nicotiana glutinosa
-
4.6.1.19 seed
-
Momordica charantia
-

Storage Stability

EC Number Storage Stability Organism
4.6.1.19 -20°C, purified enzymes, at least 2 months, no loss of activity Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 2',3'-cyclic NMP + H2O slower side reaction, intrinsic cyclic nucleotide phosphodiesterase activity Solanum lycopersicum 3'(2')-NMP
-
?
4.6.1.19 additional information besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions Solanum lycopersicum ?
-
?
4.6.1.19 additional information enzyme plays a role in self-incompatibility cell-to-cell interactions Arabidopsis thaliana ?
-
?
4.6.1.19 additional information enzyme plays a role in self-incompatibility cell-to-cell interactions Zinnia elegans ?
-
?
4.6.1.19 additional information enzyme plays a role in self-incompatibility cell-to-cell interactions Momordica charantia ?
-
?
4.6.1.19 additional information enzyme plays a role in self-incompatibility cell-to-cell interactions Nicotiana glutinosa ?
-
?
4.6.1.19 additional information enzymes show preference for release of guanine mononucleotides from diribonucleoside monophosphates or RNA, preference in descending order is G, A, U, C Solanum lycopersicum ?
-
?
4.6.1.19 nucleotidyl-3',5'-nuceotide + H2O determination of base specificity Solanum lycopersicum 2',3'-cyclic NMP + 3'(2')-NMP
-
?
4.6.1.19 RNA + H2O high-molecular weight fraction of RNA from Torula yeast, typeII-S Solanum lycopersicum 2',3'-cyclic NMP + 3'(2')-NMP 2',3'-cyclic NMPs are are further hydrolyzed in a side reaction ?

Subunits

EC Number Subunits Comment Organism
4.6.1.19 More comparison of primary, secondary, and tertiary structures, overview Arabidopsis thaliana
4.6.1.19 More comparison of primary, secondary, and tertiary structures, overview Solanum lycopersicum
4.6.1.19 More comparison of primary, secondary, and tertiary structures, overview Zinnia elegans
4.6.1.19 More comparison of primary, secondary, and tertiary structures, overview Momordica charantia
4.6.1.19 More comparison of primary, secondary, and tertiary structures, overview Nicotiana glutinosa

Synonyms

EC Number Synonyms Comment Organism
4.6.1.19 More enzymes are S-like ribonucleases Arabidopsis thaliana
4.6.1.19 More enzymes are S-like ribonucleases Zinnia elegans
4.6.1.19 More enzymes are S-like ribonucleases Nicotiana glutinosa
4.6.1.19 More enzymes belong to the RNase 1-type and are S-like RNases Solanum lycopersicum
4.6.1.19 More enzymes is a S-like ribonuclease Momordica charantia
4.6.1.19 ribonuclease LE
-
Solanum lycopersicum
4.6.1.19 RNase LE
-
Solanum lycopersicum
4.6.1.19 RNase LV-1
-
Solanum lycopersicum
4.6.1.19 RNase LV-2
-
Solanum lycopersicum
4.6.1.19 RNase LV-3
-
Solanum lycopersicum
4.6.1.19 RNase LX
-
Solanum lycopersicum
4.6.1.19 RNase MC1
-
Momordica charantia
4.6.1.19 RNase NGR3
-
Nicotiana glutinosa
4.6.1.19 RNase NW
-
Nicotiana glutinosa
4.6.1.19 RNS1
-
Arabidopsis thaliana
4.6.1.19 RNS2
-
Arabidopsis thaliana
4.6.1.19 RNS3
-
Arabidopsis thaliana
4.6.1.19 ZRNase I
-
Zinnia elegans
4.6.1.19 ZRNase II
-
Zinnia elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 37
-
assay at Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.19 100
-
5 min, different pH values and ionic strengths, complete inactivation Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 5 6 acidic optimum with yeast RNA as a substrate Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.6.1.19 Solanum lycopersicum about, RNase LV-3, isoelectric focusing
-
4
4.6.1.19 Solanum lycopersicum about, RNase LV-1, isoelectric focusing
-
4.9
4.6.1.19 Solanum lycopersicum about, RNase LV-2, isoelectric focusing
-
4.9
4.6.1.19 Solanum lycopersicum about, RNase LX, isoelectric focusing
-
4.9