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Literature summary extracted from
- Analysis of substrate recognition by the ribonucleoprotein endonuclease RNase P (2002), Methods, 28, 307-322.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | crystal structure | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.5 | Ca2+ | suppresses enzyme activity, but supports RNA folding and substrate binding, used for binding assays | Bacillus subtilis |  |
| 3.1.26.5 | EDTA | inactivation | Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.5 | additional information | - |
additional information | kinetics | Bacillus subtilis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.5 | Ca2+ | suppresses enzyme activity, but supports RNA folding and substrate binding, used for binding assays | Bacillus subtilis | |
| 3.1.26.5 | Mg2+ | best metal ion, required for folding of the RNA, for binding of protein and substrate, and for catalytic activity | Staphylococcus aureus | |
| 3.1.26.5 | Mg2+ | best metal ion, required for folding of the RNA, for binding of protein and substrate, and for catalytic activity | Bacillus subtilis | |
| 3.1.26.5 | Mg2+ | best metal ion, required for folding of the RNA, for binding of protein and substrate, and for catalytic activity | Escherichia coli | |
| 3.1.26.5 | additional information | enzyme is dependent on divalent metal ions | Escherichia coli | |
| 3.1.26.5 | additional information | enzyme is dependent on divalent metal ions, enzyme contains a metal binding loop | Staphylococcus aureus | |
| 3.1.26.5 | additional information | enzyme is dependent on divalent metal ions, enzyme contains a metal binding loop | Bacillus subtilis | |
| 3.1.26.5 | NaCl | activates | Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | 4.5S RNA precursor + H2O | Escherichia coli | RNA processing | mature 4.5S RNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | C4 antisense RNA + H2O | Escherichia coli | from bacteriophages P1 and P7 | ? | - |
? | |
| 3.1.26.5 | polycistronic mRNA precursor + H2O | Escherichia coli | RNA processing | mature mRNAs + ? | - |
? | |
| 3.1.26.5 | tmRNA precursor + H2O | Escherichia coli | RNA processing | mature tmRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | Staphylococcus aureus | tRNA processing | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | Bacillus subtilis | tRNA processing | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | Escherichia coli | tRNA processing | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA-like pseudoknotted structures in viral RNA + H2O | Escherichia coli | tRNA-like pseudoknotted structures in viral RNA | processed RNA + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.5 | Bacillus subtilis | - |
- |
- |
| 3.1.26.5 | Escherichia coli | - |
- |
- |
| 3.1.26.5 | Staphylococcus aureus | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.26.5 | endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, substrate recognition mechanism, detection methods, the RNA and protein subunits cooperate to bind different portions of the substrate structure, with the RNA subunit predominantly interacting with the mature domain of tRNA and the protein interacting with the 5'-leader sequence, substrate recognition and binding, reaction mechanism and detection methods | Bacillus subtilis |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | renaturation conditions for nearly 95% uniformly folded populations of enzyme or RNA substrate are: heating to 95°C for 3 min without divalent cation, cooling to 37°C and incubation in the presence of divalent cation for at least 15 min | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | 4.5S RNA precursor + H2O | RNA processing | Escherichia coli | mature 4.5S RNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | C4 antisense RNA + H2O | from bacteriophages P1 and P7 | Escherichia coli | ? | - |
? | |
| 3.1.26.5 | additional information | enzymatic and chemical protection, cross-linking of enzyme and substrate for determination of binding features, overview, the RNA subunit is the catalytic subunit, while the protein subunits are essential for substrate binding, broad substrate specificity | Bacillus subtilis | ? | - |
? | |
| 3.1.26.5 | additional information | the RNA subunit is the catalytic subunit, while the protein subunits are essential for substrate binding, broad substrate specificity | Staphylococcus aureus | ? | - |
? | |
| 3.1.26.5 | additional information | the RNA subunit is the catalytic subunit, while the protein subunits are essential for substrate binding, broad substrate specificity | Escherichia coli | ? | - |
? | |
| 3.1.26.5 | polycistronic mRNA precursor + H2O | RNA processing | Escherichia coli | mature mRNAs + ? | - |
? | |
| 3.1.26.5 | tmRNA precursor + H2O | RNA processing | Escherichia coli | mature tmRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | tRNA processing | Staphylococcus aureus | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | tRNA processing | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | tRNA processing | Escherichia coli | mature tRNA + 5'-oligonucleotide | - |
? | |
| 3.1.26.5 | tRNA precursor + H2O | site-specific cleavage of 5'-terminal oligonucleotide from pre-tRNA, 3 potential RNA binding motifs | Staphylococcus aureus | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| 3.1.26.5 | tRNA precursor + H2O | site-specific cleavage of 5'-terminal oligonucleotide from pre-tRNA, 3 potential RNA binding motifs | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| 3.1.26.5 | tRNA-like pseudoknotted structures in viral RNA + H2O | tRNA-like pseudoknotted structures in viral RNA | Escherichia coli | processed RNA + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | More | enzyme secondary structure, domain organization and tertiary structure, modeling, overview | Escherichia coli |
| 3.1.26.5 | More | protein subunit structure, the enzyme folds as an alpha-beta sandwich and has the overall topology of alpha(beta)3alphabetaalpha, overview | Staphylococcus aureus |
| 3.1.26.5 | More | protein subunit structure, the enzyme folds as an alpha-beta sandwich and has the overall topology of alpha(beta)3alphabetaalpha, tertiary structure, overview | Bacillus subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | RNase P | - |
Staphylococcus aureus |
| 3.1.26.5 | RNase P | - |
Bacillus subtilis |
| 3.1.26.5 | RNase P | - |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.5 | 6 | - |
assay at | Bacillus subtilis |
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