EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | gene xynB, expression as His-tagged enzyme in Escherichia coli BL21 | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.8 | additional information | - |
additional information | - |
Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Thermotoga maritima | - |
xylanase XynB, gene xynB | - |
3.2.1.8 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
xylanase XynB, gene xynB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | recombinant His-tagged enzyme from Escherichia coli, 12.5fold to homogeneity by Ni2+-chelate affinity chromatography | Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 12 | - |
substrate carboxymethylcellulose, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 30 | - |
substrate laminarin, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 64 | - |
substrate gellan gum, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 164 | - |
substrate lichenan, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 867 | - |
substrate birchwood xylan, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 1022 | - |
substrate oat spelt xylan, purified recombinant enzyme | Thermotoga maritima |
3.2.1.8 | 1099 | - |
substrate beechwood xylan, purified recombinant enzyme | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | carboxymethylcellulose + H2O | low activity, 1.4% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
3.2.1.8 | carboxymethylcellulose + H2O | low activity, 1.4% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
3.2.1.8 | gellan gum + H2O | low activity, 7.3% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
3.2.1.8 | gellan gum + H2O | low activity, 7.3% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
3.2.1.8 | laminarin + H2O | low activity, 3.4% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
3.2.1.8 | laminarin + H2O | low activity, 3.4% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
3.2.1.8 | lichenan + H2O | low activity, 19% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
3.2.1.8 | lichenan + H2O | low activity, 19% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
3.2.1.8 | additional information | substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan | Thermotoga maritima | ? | - |
? | |
3.2.1.8 | additional information | substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
3.2.1.8 | xylan + H2O | birchwood and soluble oat spelt xylan, endo-acting | Thermotoga maritima | xylobiose + xylose + ? | main degradation products | ? | |
3.2.1.8 | xylooligosaccharides + H2O | - |
Thermotoga maritima | xylobiose + xylose | predominantly xylobiose | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | xylanase B | - |
Thermotoga maritima |
3.2.1.8 | XynB | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 50 | - |
assay at | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.8 | 89.7 | - |
xylan | soluble from oat spelt, pH 6.1, 50°C | Thermotoga maritima | |
3.2.1.8 | 106.5 | - |
xylan | from birchwood, pH 6.1, 50°C | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6.1 | - |
assay at | Thermotoga maritima |