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Literature summary extracted from

  • Jiang, Z.Q.; Deng, W.; Zhu, Y.P.; Li, L.T.; Sheng, Y.J.; Hayashi, K.
    The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications (2004), J. Mol. Catal. B, 27, 207-213.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynB, expression as His-tagged enzyme in Escherichia coli BL21 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
additional information
-
Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermotoga maritima
-
xylanase XynB, gene xynB
-
3.2.1.8 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
xylanase XynB, gene xynB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged enzyme from Escherichia coli, 12.5fold to homogeneity by Ni2+-chelate affinity chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 12
-
substrate carboxymethylcellulose, purified recombinant enzyme Thermotoga maritima
3.2.1.8 30
-
substrate laminarin, purified recombinant enzyme Thermotoga maritima
3.2.1.8 64
-
substrate gellan gum, purified recombinant enzyme Thermotoga maritima
3.2.1.8 164
-
substrate lichenan, purified recombinant enzyme Thermotoga maritima
3.2.1.8 867
-
substrate birchwood xylan, purified recombinant enzyme Thermotoga maritima
3.2.1.8 1022
-
substrate oat spelt xylan, purified recombinant enzyme Thermotoga maritima
3.2.1.8 1099
-
substrate beechwood xylan, purified recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 carboxymethylcellulose + H2O low activity, 1.4% activity compared to xylan Thermotoga maritima ?
-
?
3.2.1.8 carboxymethylcellulose + H2O low activity, 1.4% activity compared to xylan Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
3.2.1.8 gellan gum + H2O low activity, 7.3% activity compared to xylan Thermotoga maritima ?
-
?
3.2.1.8 gellan gum + H2O low activity, 7.3% activity compared to xylan Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
3.2.1.8 laminarin + H2O low activity, 3.4% activity compared to xylan Thermotoga maritima ?
-
?
3.2.1.8 laminarin + H2O low activity, 3.4% activity compared to xylan Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
3.2.1.8 lichenan + H2O low activity, 19% activity compared to xylan Thermotoga maritima ?
-
?
3.2.1.8 lichenan + H2O low activity, 19% activity compared to xylan Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
3.2.1.8 additional information substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan Thermotoga maritima ?
-
?
3.2.1.8 additional information substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
3.2.1.8 xylan + H2O birchwood and soluble oat spelt xylan, endo-acting Thermotoga maritima xylobiose + xylose + ? main degradation products ?
3.2.1.8 xylooligosaccharides + H2O
-
Thermotoga maritima xylobiose + xylose predominantly xylobiose ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase B
-
Thermotoga maritima
3.2.1.8 XynB
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
assay at Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 89.7
-
xylan soluble from oat spelt, pH 6.1, 50°C Thermotoga maritima
3.2.1.8 106.5
-
xylan from birchwood, pH 6.1, 50°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.1
-
assay at Thermotoga maritima