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Literature summary extracted from

  • Ohshima, T.; Ito, Y.; Sakuraba, H.; Goda, S.; Kawarabayasi, Y.
    The Sulfolobus tokodaii gene ST1704 codes highly thermostable glucose dehydrogenase (2003), J. Mol. Catal. B, 23, 281-289.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.47 analysis enzyme can be used for glucose determination Sulfurisphaera tokodaii
1.1.1.47 biotechnology larger scale production of NAD(P)H in bioreactors by usage of the enzyme, a thermostable enzyme is advantageous Sulfurisphaera tokodaii
1.1.1.47 diagnostics enzyme can be used for glucose determination Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 gene ST1704, expression in enzyme-deficient Escherichia coli strain Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 Ca2+ 1 mM, 33% inhibition Sulfurisphaera tokodaii
1.1.1.47 Co2+ 1 mM, 44% inhibition Sulfurisphaera tokodaii
1.1.1.47 Cu2+ 1 mM, 83% inhibition Sulfurisphaera tokodaii
1.1.1.47 EDTA 1 mM, 68% inhibition, fully reversible by 20 mM MgCl2 Sulfurisphaera tokodaii
1.1.1.47 Hg2+ 1 mM, 85% inhibition Sulfurisphaera tokodaii
1.1.1.47 Mn2+ 1 mM, 41% inhibition Sulfurisphaera tokodaii
1.1.1.47 Ni2+ 1 mM, 85% inhibition Sulfurisphaera tokodaii
1.1.1.47 p-chloromercuribenzoate 1 mM, 93% inhibition Sulfurisphaera tokodaii
1.1.1.47 Zn2+ 1 mM, 25% inhibition Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 additional information
-
additional information steady-state kinetic analysis Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 Mg2+ activates by 122% at 1 mM Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 41000
-
4 * 41000, recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii
1.1.1.47 160000
-
recombinant enzyme, gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD(P)+ Sulfurisphaera tokodaii
-
D-glucono-1,5-lactone + NAD(P)H + H+
-
?
1.1.1.47 beta-D-glucose + NAD(P)+ Sulfurisphaera tokodaii 7
-
D-glucono-1,5-lactone + NAD(P)H + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Sulfurisphaera tokodaii
-
gene ST1704
-
1.1.1.47 Sulfurisphaera tokodaii 7
-
gene ST1704
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 recombinant enzyme from Escherichia coli by heat treament and 2 chromatographical steps Sulfurisphaera tokodaii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.47 D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+ sequential ordered bi-bi mechanism Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 32
-
purified recombinant enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 2-amino-2-deoxy-D-glucose + NAD(P)+
-
Sulfurisphaera tokodaii ?
-
?
1.1.1.47 2-amino-2-deoxy-D-glucose + NAD(P)+
-
Sulfurisphaera tokodaii 7 ?
-
?
1.1.1.47 2-deoxy-D-glucose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
?
1.1.1.47 2-deoxy-D-glucose + NAD(P)+ low activity Sulfurisphaera tokodaii 7 ?
-
?
1.1.1.47 6-deoxy-D-glucose + NAD(P)+
-
Sulfurisphaera tokodaii ?
-
?
1.1.1.47 beta-D-galactose + NADP+ low activity Sulfurisphaera tokodaii D-galactono-1,5-lactone + NADPH
-
?
1.1.1.47 beta-D-glucose + NAD(P)+
-
Sulfurisphaera tokodaii D-glucono-1,5-lactone + NAD(P)H + H+
-
?
1.1.1.47 beta-D-glucose + NAD(P)+
-
Sulfurisphaera tokodaii 7 D-glucono-1,5-lactone + NAD(P)H + H+
-
?
1.1.1.47 D-allose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
?
1.1.1.47 D-altrose + NADP+ low activity Sulfurisphaera tokodaii ?
-
?
1.1.1.47 D-gulose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
?
1.1.1.47 D-ribose + NADP+ low activity Sulfurisphaera tokodaii ?
-
?
1.1.1.47 D-ribose + NADP+ low activity Sulfurisphaera tokodaii 7 ?
-
?
1.1.1.47 D-xylose + NAD(P)+
-
Sulfurisphaera tokodaii ?
-
?
1.1.1.47 additional information broad substrate specificity, no activity with D-mannose, D-ribose, and glucose 6-phosphate, cofactor specificity depends on the sugar sustrate Sulfurisphaera tokodaii ?
-
?
1.1.1.47 additional information broad substrate specificity, no activity with D-mannose, D-ribose, and glucose 6-phosphate, cofactor specificity depends on the sugar sustrate Sulfurisphaera tokodaii 7 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 tetramer 4 * 41000, recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 GDH
-
Sulfurisphaera tokodaii
1.1.1.47 glucose dehydrogenase
-
Sulfurisphaera tokodaii
1.1.1.47 NAD(P)-dependent glucose-1-dehydrogenase
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 75
-
-
Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 additional information
-
highly thermostable enzyme Sulfurisphaera tokodaii
1.1.1.47 80
-
15 min, more than 90% remaining activity, pH 8.0 Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 9
-
-
Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.47 4.5 11 50% of maximal activity at pH 4.5 and pH 11.0 Sulfurisphaera tokodaii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.47 5 10.5 stable at 37°C for 30 min Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
Sulfurisphaera tokodaii
1.1.1.47 NADH
-
Sulfurisphaera tokodaii
1.1.1.47 NADP+
-
Sulfurisphaera tokodaii
1.1.1.47 NADPH
-
Sulfurisphaera tokodaii