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Literature summary extracted from

  • Chan, S.; Segelke, B.; Lekin, T.; Krupka, H.; Cho, U.S.; Kim, M.Y.; So, M.; Kim, C.Y.; Naranjo, C.M.; Rogers, Y.C.; Park, M.S.; Waldo, G.S.; Pashkov, I.; Cascio, D.; Perry, J.L.; Sawaya, M.R.
    Crystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism (2004), J. Mol. Biol., 341, 503-517.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 medicine potential target for anti-tuberculosis therapy Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21-Gold (DE3) as His-tag fusion protein Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 hanging and sitting drop vapor diffusion method Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required for stabilization of the active form of the enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis H37Rv removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Mycobacterium tuberculosis P9WNS5
-
-
3.6.1.23 Mycobacterium tuberculosis H37Rv P9WNS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant protein using His-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?