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Literature summary extracted from

  • Tanaka, N.; Arai, J.; Inokuchi, N.; Koyama, T.; Ohgi, K.; Irie, M.; Nakamura, K.T.
    Crystal structure of a plant ribonuclease, RNase LE (2000), J. Mol. Biol., 298, 859-873.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.19 20 mg/ml purified recombinant wild-type enzyme, hanging drop vapour diffusion method, equal volumes of protein solution, 20 mM citrate, pH 5.0, and reservoir solution, 0.1 M citrate, pH 5.0, 36% w/v PEG 1540, X-ray diffraction structure determination and analysis at 1.65-2.2 A resolution of wild-type enzyme crystals and heavy atom derivatives Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.19 H109F site-directed mutagenesis, 35% activity compared to the wild-type enzyme Solanum lycopersicum
4.6.1.19 H134F site-directed mutagenesis, 49% activity compared to the wild-type enzyme Solanum lycopersicum
4.6.1.19 H39F site-directed mutagenesis, conserved active site residue, 0.18% activity compared to the wild-type enzyme Solanum lycopersicum
4.6.1.19 H92F site-directed mutagenesis, conserved active site residue, 0.21% activity compared to the wild-type enzyme Solanum lycopersicum
4.6.1.19 H97F site-directed mutagenesis, conserved active site residue, 0.12% activity compared to the wild-type enzyme Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.19 24000
-
 x * 24000, about Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Solanum lycopersicum P80022
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.19 RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] catalytic hydrolysis mechanism, active site structure, the conserved active site residues His39, His92, and His 97 are essential for activity, Glu93 and Lys96 are important Solanum lycopersicum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.19 cell culture
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 additional information broad substrate specificity Solanum lycopersicum ?
-
 ?
4.6.1.19 RNA + H2O
-
 Solanum lycopersicum nucleoside 3'-phosphates and 3'-phosphooligonucleotides via oligonucleotides or mononucleotides with 2',3'-cyclic phosphate at the 3'-side ?

Subunits

EC Number Subunits Comment Organism
4.6.1.19 ? x * 24000, about Solanum lycopersicum
4.6.1.19 More disulfide bridge pattern Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
4.6.1.19 acid ribonuclease
-
 Solanum lycopersicum
4.6.1.19 acid RNase
-
 Solanum lycopersicum
4.6.1.19 base-non-specific ribonuclease
-
 Solanum lycopersicum
4.6.1.19 Escherichia coli ribonuclease I' ribonuclease PP2'
-
 Solanum lycopersicum
4.6.1.19 Escherichia coli ribonuclease II
-
 Solanum lycopersicum
4.6.1.19 More enzyme belongs to the ribonuclease T2 family, structure comparison and amino acid sequence alignment Solanum lycopersicum
4.6.1.19 non-base specific ribonuclease
-
 Solanum lycopersicum
4.6.1.19 nonbase-specific RNase
-
 Solanum lycopersicum
4.6.1.19 nonspecific RNase
-
 Solanum lycopersicum
4.6.1.19 ribonnuclease (non-base-specific)
-
 Solanum lycopersicum
4.6.1.19 ribonuclease (non-base-specific)
-
 Solanum lycopersicum
4.6.1.19 ribonuclease 3'-oligonucleotide hydrolase
-
 Solanum lycopersicum
4.6.1.19 ribonuclease II
-
 Solanum lycopersicum
4.6.1.19 ribonuclease LE
-
 Solanum lycopersicum
4.6.1.19 ribonuclease M
-
 Solanum lycopersicum
4.6.1.19 ribonuclease N2
-
 Solanum lycopersicum
4.6.1.19 ribonuclease nucleotido-2'-transferase (cyclizing)
-
 Solanum lycopersicum
4.6.1.19 ribonuclease PP3
-
 Solanum lycopersicum
4.6.1.19 ribonuclease U4
-
 Solanum lycopersicum
4.6.1.19 RNase (non-base specific)
-
 Solanum lycopersicum
4.6.1.19 RNase CL
-
 Solanum lycopersicum
4.6.1.19 RNase II
-
 Solanum lycopersicum
4.6.1.19 RNase LE
-
 Solanum lycopersicum
4.6.1.19 RNase M
-
 Solanum lycopersicum
4.6.1.19 RNase Ms
-
 Solanum lycopersicum
4.6.1.19 RNase T2
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 37
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 5.5
-
-
 Solanum lycopersicum