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Literature summary extracted from
- Overexpression, purification, and biochemical characterization of the thermostable NAD-dependent alcohol dehydrogenase from Bacillus stearothermophilus (2003), J. Microbiol. Biotechnol., 13, 738-744.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | biotechnology | possible usage of the enzyme in bioindustrial processes and as biosensor | Geobacillus stearothermophilus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | overexpression as GST-fusion protein in Escherichia coli | Geobacillus stearothermophilus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.1 | highly stable against 0.1 M urea and 0.05% SDS | Geobacillus stearothermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | imidazole | - |
Geobacillus stearothermophilus |  |
| 1.1.1.1 | Pyridine | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 5.11 | - |
ethanol | - |
Geobacillus stearothermophilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | reombinant fusion enzyme by glutathione affinity chromatography, cleavage of GST fusion tag by thrombin, further purification of the active enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | ethanol + NAD+ | - |
Geobacillus stearothermophilus | acetaldehyde + NADH | - |
? | |
| 1.1.1.1 | additional information | broad substrate specificity | Geobacillus stearothermophilus | ? | - |
? | |
| 1.1.1.1 | secondary alcohol + NAD+ | - |
Geobacillus stearothermophilus | aldehyde + NADH | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | ADH | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 70 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 68 | - |
purified recombinant enzyme, most stable at | Geobacillus stearothermophilus |
| 1.1.1.1 | 85 | - |
remaining activity | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 9 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | NAD+ | dependent on | Geobacillus stearothermophilus | |
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Release 2023.1 (January 2023)
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