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Literature summary extracted from

  • Dey, S.; Hu, Z.; Xu, X.L.; Sacchettini, J.C.; Grant, G.A.
    D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis is a link between the E. coli and mammalian enzymes (2005), J. Biol. Chem., 280, 1484-14891.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.95
-
 Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
1.1.1.95 at low ionic strength the enzyme irreversibly loses activity with time. In 20 mM phosphate buffer, pH 7.5, most of the activity is lost within 24 h. The activity loss is prevented if the ionic strength is kept above approximately 100 mM salt Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.95 L-serine I0.5: 0.03 mM. In presence of KCl, the binding and the inhibition of L-serine, are cooperative and in the absence of KCl they are not Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.95 0.085
-
 3-phosphohydroxypyruvate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.95 potassium phosphate optimal activity at 75-100 mM, only about 30% of the optimal activity in 5 mM potassium phosphate Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.95 55000
-
 x * 55000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.95
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phosphohydroxypyruvate + NADH specific for Mycobacterium tuberculosis 3-phosphoglycerate + NAD+
-
 ?
1.1.1.95 additional information no activity with alpha-ketoglutarate Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.95 ? x * 55000, SDS-PAGE Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.95 6.5
-
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.95 NADH
-
 Mycobacterium tuberculosis