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Literature summary extracted from
- A pyrophosphatase regulating polyphosphate metabolism in acidocalcisomes is essential for Trypanosoma brucei virulence in mice (2004), J. Biol. Chem., 279, 3420-3425.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.1.3.1 | expressed in Escherichia coli BL21(DE3) | Trypanosoma brucei |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | Ca2+ | competitive | Trypanosoma brucei |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.3.1 | 0.014 | - |
Polyphosphate | in the presence of Zn2+, pH 7.5, 25°C | Trypanosoma brucei | |
| 7.1.3.1 | 0.019 | - |
diphosphate | in the presence of Mg2+, pH 7.5, 25°C | Trypanosoma brucei | |
| 7.1.3.1 | 0.373 | - |
tripolyphosphate | in the presence of Zn2+, pH 7.5, 25°C | Trypanosoma brucei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.3.1 | acidocalcisome | soluble | Trypanosoma brucei | 20022 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | Ca2+ | CaCl2 at 0.1 mM favors the formation of large complexes | Trypanosoma brucei | |
| 7.1.3.1 | Mg2+ | required | Trypanosoma brucei | |
| 7.1.3.1 | Zn2+ | required for the hydrolysis of tripolyphosphate and polyphosphate | Trypanosoma brucei | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.3.1 | 47300 | - |
calculated from predicted amino acid sequence | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O | Trypanosoma brucei | important for energy metabolism, provides energy for biosynthetic reactions, essential for growth of bloodstream forms in mammalian host | 2 phosphate | - |
? | |
| 7.1.3.1 | polyphosphate + H2O | Trypanosoma brucei | polyphosphate of 28 residues, hydrolyzed only in the presence of Zn2+, central role in the regulation of polyphosphate metabolism, supports recovery from hypoosmotic stress | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.3.1 | Trypanosoma brucei | Q7Z029 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.3.1 | recombinant protein using His-tag | Trypanosoma brucei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O | - |
Trypanosoma brucei | 2 phosphate | - |
? | |
| 7.1.3.1 | diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions, essential for growth of bloodstream forms in mammalian host | Trypanosoma brucei | 2 phosphate | - |
? | |
| 7.1.3.1 | polyphosphate + H2O | polyphosphate of 28 residues, hydrolyzed only in the presence of Zn2+, central role in the regulation of polyphosphate metabolism, supports recovery from hypoosmotic stress | Trypanosoma brucei | ? | - |
? | |
| 7.1.3.1 | polyphosphate + H2O | polyphosphate of 28 residues, poor substrate, hydrolyzed only in the presence of Zn2+ | Trypanosoma brucei | ? | - |
? | |
| 7.1.3.1 | tripolyphosphate + H2O | hydrolyzed only in the presence of Zn2+ | Trypanosoma brucei | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.3.1 | acidocalcisomal pyrophosphatase | - |
Trypanosoma brucei |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.3.1 | 3.5 | - |
Polyphosphate | in the presence of Zn2+, pH 7.5, 25°C | Trypanosoma brucei | |
| 7.1.3.1 | 52 | - |
tripolyphosphate | in the presence of Zn2+, pH 7.5, 25°C | Trypanosoma brucei | |
| 7.1.3.1 | 56 | - |
diphosphate | in the presence of Zn2+, pH 7.5, 25°C | Trypanosoma brucei | |
| 7.1.3.1 | 385 | - |
diphosphate | in the presence of Mg2+, pH 7.5, 25°C | Trypanosoma brucei | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.3.1 | 6.5 | - |
in the presence of Zn2+ | Trypanosoma brucei |
| 7.1.3.1 | 7.5 | - |
in the presence of Mg2+ | Trypanosoma brucei |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.3.1 | 0.014 | - |
Ca2+ | hydrolysis of diphosphate, pH 7.5, 25°C, no inhibition of tripolyphosphate and polyphosphate hydrolysis | Trypanosoma brucei | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 7.1.3.1 | Trypanosoma brucei | calculated from predicted amino acid sequence | - |
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