EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in different Escherichia coli strains | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | hanging and sitting drop vapor diffusion method, stable monomers observed in crystal phase | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.23 | DELTA160-187 | truncated form | Drosophila melanogaster |
EC Number | General Stability | Organism |
---|---|---|
3.6.1.23 | dUMP protects against tryptic digestion, slight fragmentation caused by frequent freezing and thawing that does not affect the activity | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | dUDP | - |
Drosophila melanogaster | |
3.6.1.23 | dUMP | - |
Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0004 | - |
dUTP | pH 7.5, 25°C | Drosophila melanogaster | |
3.6.1.23 | 0.64 | - |
dTTP | pH 7.5, 25°C | Drosophila melanogaster | |
3.6.1.23 | 2.3 | - |
dCTP | pH 7.5, 25°C | Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required, modulates conformation of the enzyme | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 17200 | - |
3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
3.6.1.23 | 19800 | - |
3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
3.6.1.23 | 54000 | - |
truncated enzyme, gel filtration | Drosophila melanogaster |
3.6.1.23 | 65000 | - |
full length enzyme, gel filtration | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Drosophila melanogaster | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | recombinant protein | Drosophila melanogaster |
EC Number | Storage Stability | Organism |
---|---|---|
3.6.1.23 | -70°C, stable for at least 3 months | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dCTP + H2O | - |
Drosophila melanogaster | dCMP + diphosphate | - |
? | |
3.6.1.23 | dTTP + H2O | - |
Drosophila melanogaster | dTMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Drosophila melanogaster | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis | Drosophila melanogaster | dUMP + diphosphate | - |
? | |
3.6.1.23 | additional information | no hydrolysis of dUDP | Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | trimer | 3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 54 | - |
melting point | Drosophila melanogaster |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.1 | - |
dTTP | pH 7.5, 25°C | Drosophila melanogaster | |
3.6.1.23 | 0.7 | - |
dCTP | pH 7.5, 25°C | Drosophila melanogaster | |
3.6.1.23 | 12 | - |
dUTP | pH 7.5, 25°C | Drosophila melanogaster |